胶质细胞源性神经营养因子(GDNF)在不同年龄公猪睾丸中的表达  

Expression of Glial Cell Line-derived Neurotrophic Factor(GDNF)in Boar Testes during Different Stages

作　　者：孙燕[1] 王鲜忠[1] 吴建云[1] 周沛[2] 宋艳画[1] 张家骅[1] 

机构地区：[1]西南农业大学重庆市牧草与草食家畜重点实验室,重庆400716 [2]西南农业大学动物科技学院,重庆400716

出　　处：《农业生物技术学报》2005年第6期763-767,i0010,共6页Journal of Agricultural Biotechnology

基　　金：教育部博士学科点专项基金(No.20040625004);国家自然科学基金(No.30270955)资助

摘　　要：采用免疫组化(immunohistochemistry)和半定量RT-PCR法研究了胶质细胞源性神经营养因子(Glialcellline-derivedneurotrophicfactor,GDNF)在生后仔猪睾丸中的表达,探讨了GDNF在睾丸发育和精子发生中的作用。实验结果发现,在幼龄阶段(2～4周龄),GDNF蛋白主要表达于间质细胞区,而在曲细精管内未见表达。从第2月龄开始,GDNF蛋白在曲细精管内的支持细胞胞浆出现阳性信号,呈较低水平表达。随着年龄的进一步增长,GDNF在睾丸曲细精管内的表达不断增强,到成年时GDNF蛋白表达水平达最高。RT-PCR结果显示,仔猪出生后2周,睾丸中即存在GDNFmRNA的表达,随着年龄的增加,GDNFmRNA水平持续升高,到2月龄和6、18月龄分别与出生2周时有显著性(P<0.05)和极显著性(P<0.01)差异,其中第6月龄达高峰。实验结果表明GDNF可能直接参与了出生后仔猪睾丸的发育,并在调节精子发生中起着重要的作用。Using semi-quantity RT-PCR and immunohistochemistry to assay the expression of Glial cell line-derived neurotrophic factor（GDNF） in boar testes during different stages and to investigate the significance of GDNF in the regulation of testes development and spermatogenesis. In the immunostaining slides, GDNF was found positively expressed in the Leydig cell area from 2 to 4 weeks, and was found positive expressed in Leydig cells and in the seminiferous tubules after 2 months old. Results from RT-PCR indicated that GDNF gene expressed in the testes, showing the strong postivie band in the PCR product electrophoresis gel. These results showed that GDNF plays a direct role in the regulation of testicular development and spermatogenesis.

关 键 词：仔猪 睾丸 胶质细胞源性神经营养因子 免疫组化 RT-PCR 

分 类 号：S896.4[农业科学—特种经济动物饲养] Q421[农业科学—畜牧兽医]