桃ACO基因反义转化桃幼胚子叶的研究  

Genetic Transformation of Peach Immature Cotyledons with Its Antisenes ACO Gene

作　　者：吴延军[1] 张上隆[1] 谢鸣[2] 陈俊伟[2] 蒋桂华[2] 秦永华[1] 秦巧平[1] 

机构地区：[1]浙江大学农业与生物技术学院园艺系,杭州310029 [2]浙江省农业科学院园艺所,杭州310021

出　　处：《遗传》2006年第1期65-70,共6页Hereditas（Beijing)

基　　金：宁波市科委项目支持桃转基因研究(2000农业项目支助);浙江省农业科学院科研启动项目资助~~

摘　　要：用根癌农杆菌(Agrobacterium tum efaciens)和基因枪两种方法,以桃(Prunus persicaL.Batsch)幼胚子叶为受体,转化桃ACC氧化酶(ACO)反义基因片段,经筛选培养获得了卡那霉素抗性芽。微芽嫁接培养抗性芽部分可成株。PCR、Southern杂交和GUS基因表达等分子检测,初步表明外源反义ACO基因已经整合到桃基因组中。Genetic transformation of peach immature cotyledons with its ACO antisense gene was studied by using particle bombardment method through Agrobacterium tumefaciens. Kanr shoots and Kanr plantiet were obtained. The plantet with ACO antisenes gene through Agrobacterium tumefaciens was obtained by micrografting technique and survived for nearly one month. The results of the PCR, POR-southern, genomes southern hybridization analysis and GUS color reaction of some Kan＇ materials showed in some degree that the peach ACO antisens gene was integrated into peach genomes.

关 键 词：根癌农杆菌 基因枪 转基因 反义ACO基因 

分 类 号：S662.1[农业科学—果树学]