黄淮中北部大豆花叶病毒株系的鉴定与分布  

Identification and distribution of soybean mosaic virus strains in Middle and Northern Huang Huai Region of China

作　　者：郭东全[1] 智海剑[1] 王延伟[1] 盖钧镒[1] 周新安[2] 杨崇良[3] 李凯[1] 李海朝[1] 

机构地区：[1]南京农业大学大豆研究所国家大豆改良中心作物遗传与种质创新国家重点实验室,江苏南京210095 [2]中国农业科学院油料作物研究所,湖北武汉430062 [3]山东省农业科学院植保所,山东济南250100

出　　处：《中国油料作物学报》2005年第4期64-68,共5页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金资助项目(30170607)

摘　　要：2002-2003年采集了黄淮中北部地区为主的32个县市的大豆SMV病样981份,经初步繁殖鉴定、生物纯化及DAS-ELISA血清学检测,得到95个SMV分离物,根据95个分离物在27个鉴别寄主上的症状反应,最终确定南农1138-2、诱变30、8101、铁丰25、Davis、Bullfalo、早熟18、Kwanggyo、齐黄1号和科丰1号10个品种作为该地区SMV株系的鉴别寄主.利用它们可将95个SMV分离物划分成10个株系群,其中5个与以往鉴定株系群相同,另5个为新发现的株系群,分别将5个新株系群定名为SC-11～SC-15.研究还发现,SC-11株系群所占比例最大,是本地区优势株系;其次为SC-8株系群;SC-11和SC-13株系群分布最广,在黄淮北部各省均有分布.Nine hundred and eighty-one diseased specimens of soybean leaves were collected from Middle and Northern Huang Huai Region in 2002 and 2003, and 95 SMV isolates were obtained using biological purification and serology （DAS-ELISA）. Based on symptomatic response of 27 differential hosts to these strains, Nannong 1138-2, Youbian 30, 8101 ,Tiefeng 25, Davis, Bullfalo, Zaoshou 18, Kwanggyo, Qihuang 1 and Kefeng 1 were chosen as the differential hosts for the identification of the SMV strain groups in Northern Huang Huai Region of China. The 95 SMV isolates were classified into 10 strain groups. Five new strain groups were discovered and named SC-11-SC-15. In Northern Huang Huai Region, SC-11 was preponderant, accounting for 21.1%. The second main strain group was the SC-8, accounting for 17.9%. The strain groups with the widest distribution were SC-11 and SC-13, which can be detected in all provinces of the region.

关 键 词：大豆花叶病毒 株系鉴定 株系分布 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治] S332.2[农业科学—植物保护]