近江牡蛎肠道细菌及其产酶能力的研究  

Intestine Bacteria from Jinjiang Ostrea vivularis Gould and Its Zymogenicities

作　　者：杨吉霞[1] 蔡俊鹏[1] 余德民[1] 

机构地区：[1]华南理工大学食品与生物工程学院,广东广州510640

出　　处：《微生物学杂志》2005年第5期26-28,共3页Journal of Microbiology

基　　金：广东省自然科学基金重点项目(020964);华南理工大学"高水平大学建设"苗子项目

摘　　要：从近江牡蛎(Jinjiang Ostrea vivularisGould)肠道中分离出21株菌,其中9株革兰氏阳性菌,12株革兰氏阴性菌。并检测其蛋白酶、脂肪酶、淀粉酶、纤维素酶的产酶能力。结果表明,13株菌(61.9%)能分泌蛋白酶,13株(61.9%)分泌淀粉酶;11株(52.4%)产脂肪酶;7株(33.3%)产纤维素酶。产4种酶的有5株,产3种酶的3株,产2种酶的5株,产1种酶的3株,不产酶的仅有5株。产酶菌株的比例高达76.2%(16/21),可见近江牡蛎肠道细菌对食饵消化有重要作用。21 strains bacteria were isolated from Jinjiang Ostrea vivularis Gould （JOV）, among them 9 were Gram positive, and 12 were Gram negative. Their zymogenicities of protease, lipase, amylase, and cellulase were measured. The results showed that 13 strains （61,9%） were able to produce extra-cellular protease and amylase, 11 strains （52.4 % ） excrete lipase, 7 strains （33.3 % ） cellulase. Among them 5 strains excrete four kinds of enzyme, 3 strains three kinds of enzyme, 5 strains two kinds of enzyme, and 3 strains one enzyme. And only 5 strains produce no enzyme. The rate of enzyme-producing strain is as high as 76.2 % （16/21）, Therefore, it is clear that bacteria in intestine of JOV play an important role in the digestion of food.

关 键 词：近江牡蛎(Jinjiang Ostrea vivularis Gould) 肠道细菌 胞外酶 

分 类 号：S944.41[农业科学—水产养殖]