提高大白菜游离小孢子胚诱导率的研究  

A Study on lmproving Frequency of Isolated Microspore-derived Embryos of Chinese Cabbages

作　　者：蒋武生[1] 原玉香[1] 张晓伟[1] 耿建峰[1] 韩永平[1] 高睦枪[1] 栗根义[1] 

机构地区：[1]河南省农业科学院生物技术研究所,河南郑州450002

出　　处：《华北农学报》2005年第6期34-37,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家863资助项目(2001AA241125);河南省重点攻关项目(0123012200)

摘　　要：采用游离小孢子培养方法,对提高大白菜小孢子胚诱导和形成的因素进行了研究.结果表明,24个大白菜品种(品系)在NLN-13液体培养基中有21个品种诱导出胚.各品种间小孢子胚产量差别极大,每花蕾产胚在0.1～14.66个之间.比较添加不同浓度蔗糖和激素的NLN培养基的培养效果,以NLN+13%蔗糖的效果较好.接种后,小孢子在33℃高温预处理24～48 h,产胚量较高.B5+6-BA 0.2 mg/L+3%蔗糖+1%琼脂培养基有利于小孢子胚长成植株.Studies on factors for enhancing the embryogenic rates in isolated microspore culture of chinese cabbages were carried out. The results showed that 21 varieties had produced microspore-derived embryos among 24 varieties of chinese cabbage cultured in NLN-13 liquid medium.The production of microspore-derived embryos varied remarkably among these varieties. Embryo yield per bud is from 0.1 to 14.66. Sucrose and hormone concentrations in NLN medium were compared. NLN with 13% sucrose is better in production of microspore-derived embryos. Average embryo yield is higher in 33℃ high temperature treatment from 24 to 48 hour. The B5 medium with 6-BA 0.2 mg/L, 3 % Sucrose and 1% agar is good for microspore-derived embryo to regenerated plants.

关 键 词：大白菜 游离小孢子培养 诱导率 小孢子胚 

分 类 号：S634.1[农业科学—蔬菜学]