多发梗塞性痴呆大鼠海马抗氧化酶活性的变化及针刺的干预作用  

On Changes of Activity of Antioxidases in Hippocampus of Rats with Multi-Infarct Dementia and the Intervention Effects of Acupuncture

作　　者：刘存志[1] 于建春[1] 张雪竹[1] 王彤[2] 韩景献[1] 

机构地区：[1]天津中医学院第一附属医院老年病研究室,天津300193 [2]天津中医学院,天津300193

出　　处：《中华中医药杂志》2005年第12期724-726,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.30472242);天津市自然科学基金(No.033606811)

摘　　要：目的:探讨多发梗塞性痴呆大鼠脑内抗氧化酶活性的变化及针刺的干预作用。方法:通过栓子注入法制作多发梗塞性痴呆模型,随机分为模型组、针刺组和非穴组,并设正常组和假手术组作对照。观察各组大鼠海马区丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性的变化情况。结果:与正常组比较,模型组海马MDA含量明显升高,SOD及GSH-Px活性明显下降,CAT活性无明显变化。针刺可明显逆转上述异常改变,与正常组比较无显著差异。结论:SOD/GSH-Px途径在多发梗塞性痴呆大鼠脑内抗氧化体系中占居主导地位,CAT可能不是脑内主要的抗氧化酶。针刺通过提高SOD/GSH-Px活性在脑内发挥抗氧化作用,这可能是其治疗血管性痴呆的重要机制。Objective: To explore the changes of activity of antioxidases in hippocampus of the rats with multi-infarct dementia and the intervention effects of acupuncture.Method: The multi-infarct dementia model was made by injected emboli into the internal carotid artery.The demented rats were allocated to 3 groups randomly: model,acup and sham-acup;meanwhile normal and sham-operated rats were used as control.The parameters of oxidative stress were assessed by measuring the malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT) and glutathione peroxidase(GSH-Px) levels in hippocampus after acupuncture treatment.Result: Compared to normal group,MDA levels of hippocampus in model animals increased significantly,while SOD and GSH-Px decreased much more;No significant difference was observed in CAT among groups.Interestingly,the acupuncture treatment could significantly improve all those indexes.Conclusion: SOD/GSH-Px pathway may be playing a major role in combating oxidative stress in the brain of MID rats,the CAT activity not the key enzyme.Acupuncture treatment could increase the activity of antioxidase SOD/GSH-Px,alleviate the injury of free radical and maintain the oxidant-antioxidant balance to a great extent.This may be one of the mechanisms of its therapeutic effect.

关 键 词：血管性痴呆 针刺 氧化应激 实验研究 

分 类 号：R245[医药卫生—针灸推拿学]