用ro/B基因转化大红甜橙的初步研究  

Genetic Transformation of ‘Dahong’ Sweet Orange with rolB Gene

作　　者：敖小平[1] 胡新喜[1] 郭琛[1] 焦徕[1] 邓子牛[1] 熊兴耀[1] 

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2005年第6期623-626,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家转基因专项资助项目(YJ04-B-02);湖南省科技厅重大科技专项(04NK1005)

摘　　要：为建立大红甜橙的再生及遗传转化体系,提高转化芽的成株率,以30d龄的大红甜橙(CitrussinensisOsbeck)实生苗上胚轴为材料,导入rolB基因,进行遗传转化研究.结果表明:MS+3.0mg/L6-BA培养基诱导不定芽的效果最好,达95%以上.遗传转化时,采用培养基Ⅱ(MS+3mg/L6-BA+200μmol/LAs)+培养基Ⅳ(MT+3mg/L6-BA+50mg/LKan+500mg/LCef)组合的抗性芽诱导最快,诱导率为51%.用微芽嫁接的方法,将抗性芽嫁接到卡里佐枳橙上,成苗良好,已获得19株转rolB基因植株,盆栽在温室中生长良好.Internodal stem segments from 30 d old seedlings of ‘Dahong＇ sweet orange（Citrus sinensis Osbeck） were cultured on bud induction media, to study on the genetic transformation in Citrus with rolB gene. The best medium for induction was MS＋3.0 mg/L 6-BA, which led to 95% of explants producing at least one bud, so this medium could be considered as the best for bud induction in plant regeneration. The co-culture medium Ⅱ （MS＋3 mg/L 6-BA＋200 μmol/L As）＋selective medium Ⅳ （MT＋3 mg/L 6-BA＋50 mg/L Kan＋500 mg/L Cef） were the suitable combination for production of transgenic bud, and on which the ratio of buds induction could be 51%. Kan-resistant buds were micro grafted on in vitro grown seedlings of C. SinensisxP. trifoliata, and 19 transgenic plants derived there well in the greenhouse.

关 键 词：大红甜橙 遗传转化 ROLB基因 

分 类 号：S666.4[农业科学—果树学]