HPLC法测定盐酸雷尼替丁胶囊的含量  

Determination of Ranitidine Hydrochloride Capsules by HPLC

作　　者：施友玲[1] 

机构地区：[1]福建省药品检验所,福州350001

出　　处：《海峡药学》2005年第6期69-71,共3页Strait Pharmaceutical Journal

摘　　要：目的测定盐酸雷尼替丁胶囊含量.方法采用HPLC法,色谱柱为Hypersil ODS C18(250×4.0mm,5μm),流动相为甲醇-0.77%醋酸铵溶液(70 :30),测定波长320nm.结果线性范围4.4～66.0μg·mL-1,r=0.9999;平均加样回收率为100.85%,RSD=0.63%(n=6).结论方法简便、重现性好,准确可靠.OBJECTIVE To establish HPLC method for the determination of ranitidine Hydrochloride capsules. METHODS An HPLC method was chromato graphed on C18（4. 6mm×250mm,5μm ） columns with a mobile phase consisted of mixer of CH3OH：CH3COONH4 （0.1 mol · L^-1） （42.58）. The flow rate was 1 mL · min^-1. The detection was carried out with an ultraviolet detector at 320nm. The injection volumewas 20μL . Ranitidine Hydrochloride was measured with the external standard method. RESULTS The regression equation was A=45. 162C＋1. 9614,r =0. 9999 ; The linear range of was 15.8-59.3μg · mL^-1 ,The average recovery was 100. 1% , RSD was 0. 43%. CONCLUSION The method sensitive and accurate ; It can be used for the quantitative determination of ranitidine hydrochloride and its capsules.

关 键 词：盐酸雷尼替丁胶囊 HPLC法 含量测定 

分 类 号：R927.2[医药卫生—药学]