RP-HPLC法测定牡丹皮和杞菊地黄丸中丹皮酚的含量  

Determination of the contents of Paeonol in Cortex Muotan and Qijudihuang Pills by RP-HPLC

作　　者：梁贵键[1] 朱莉[1] 张华[1] 张蔚君[1] 张福成[1] 吕冬 端裕树 

机构地区：[1]空军总医院药学部,北京100036 [2]北京岛津分析中心,北京100020

出　　处：《空军总医院学报》2005年第4期206-207,210,共3页Journal of General Hospital of Air Force,PLA

摘　　要：目的建立牡丹皮和杞菊地黄丸中丹皮酚含量测定方法.方法采用HPLC法测定杞菊地黄丸中丹皮酚的含量,固定:CLO-ODS柱(150 mm×4.6 mm),流动相:甲醇-磷酸缓冲盐(pH3.1)(42:58),检测波长299 nm,柱温30℃,流速1.0 ml/min,丹皮酚保留时间7.5 min.结果牡丹皮和杞菊地黄丸中的丹皮酚与其他成分分离良好,丹皮酚线性范围5～50 μg/L,r=0.9997(n=6).牡丹皮药材中丹皮酚平均回收率为97.86%,RSD=0.64%.结论本法分析简便、灵敏、迅速、准确.Objective To establish a method for the quantitative determination of the contents of Paeonol in Cortex Muotan and Qijudihuang Pills. Methods Paeonol were separated and determined by reversed phase high performance liquid chromatography （RP-HPLC）, with a CLO-ODS column （150× 4. 6mm） and a mixture of methanol-phosphate buffer（pH3. 1）（42 ： 58）as mobile phase（particle size 5 microns）. The flow rate was 1 ml/min. The column temperature was 30℃. The detection wavelength was 299 nm. The retention time for Paeonol was 7. 5 min. Results Paeonol in Cortex Muotan has a good linearity,and the average recovery with RSD （n：6）is 97. 86±0. 64% for paeonol. The linear calibration curve for Paeonol was obtained in the range 5.0 ～50.0 micrograms with the correlation coefficient 0. 9997, Conclusion The method is simple,sensitive,rapid and accurate.

关 键 词：色谱法 高压液相 丹皮酚/分析 牡丹皮/分析 杞菊地黄汤/分析 

分 类 号：R283.6[医药卫生—中药学] R289.5[医药卫生—中医学]