不同来源扶芳藤药材的HPLC指纹图谱比较  

Comparison of HPLC Fingerprint Pictures between Euonymus fortunei from Different Resources

作　　者：叶勇[1] 覃洁萍[1] 李芸[2] 杨玲芳[3] 

机构地区：[1]广西中医学院,广西南宁530001 [2]广西中医学院制药厂,广西南宁530023 [3]广西中医学院中药专业,2004届实习生广西南宁530001

出　　处：《广西中医学院学报》2005年第4期53-56,共4页Journal of Guangxi Traditional Chinese Medical University

基　　金：广西自然科学基金资助项目(桂科自0447076)

摘　　要：[目的]应用高效液相法测定不同来源扶芳藤药材HPLC指纹图谱,并对其HPLC指纹图谱进行比较。[方法]用shim-pack CLC-ODS(6.0mmID×15cm)色谱柱,0.05%磷酸和乙腈梯度洗脱,流速1.0ml/min,柱温25℃,检测波长203nm。[结果]不同来源的扶芳藤药材HPLC指纹图谱有较明显的差异。[结论]固定药材的来源和前处理方法是保证药品质量一致性的前提。[Objective]To determine the fingerprint pictures of Euonymus fortunei from different resources by HPLC method, and then make a comparison between them. [Methods] Shim-pack CLC-ODS （6.0mmID × 15cm） column was applied with 0.05% Phosphoric acid and acetonitrile as mobile phase in a gradient mode. The flow. rate was 1.0 ml/min, the temperature of column 25℃ , and the wave-length of detection 203 nm. [Results] The fingerprint pictures of Euonymus fortunei from different resources showed the different characteristics. [Conclusion]The resource and the preparation of crude drug will have great effects on the quality of corresponding medicinal products.

关 键 词：扶芳藤 指纹图谱 梯度洗脱 HPLC法 

分 类 号：R284[医药卫生—中药学]