高效毛细管电泳法测定石榴皮中鞣花酸的含量  

Content Determination of Ellagic Acid in Granati Cortex by HPCE

作　　者：周本宏[1] 吴振华[2] 李小军[2] 刘春[2] 张杰[1] 

机构地区：[1]武汉大学人民医院,武汉市430060 [2]武汉大学药学院,武汉市430072

出　　处：《中国药房》2005年第24期1893-1894,共2页China Pharmacy

基　　金：国家自然科学基金资助项目(30370157)

摘　　要：目的:建立以高效毛细管电泳法测定石榴皮提取物中鞣花酸含量的方法.方法:毛细管柱为未涂层毛细管,缓冲液为30mmol/L氨基丁三醇-30mmol/L磷酸二氢钾(20:9),分离电压为20kV,检测波长为254nm,柱温为25℃,进样条件为25mbar、5.0s.结果:鞣花酸检测浓度在0.0 398～0.3 184mg/ml范围内与峰面积积分值线性关系良好(r=0.9 993);平均加样回收率为97.42%(RSD=1.84%).结论:本方法准确、可靠,可用于石榴皮药材的质量控制.OBJECTIVE： To establish a method of high- performance capillary electrophoresis（HPCE） for content deterruination of ellafic acid in granati cortex.METHODS：The capillary pillar used in this study was capillary without smear layer, the buffer solution was 30mmol/L tromethamine- 30mmol/L potassium dihydrogen phosphate（20 ： 9）, the running voltage was 20kV, the wavelength of detection was 254nm, the column temperature was 25℃, and the sampling condition was 25mbar, 5.0s. RESULTS：The detected concentration of ellagic acid showed a good linear relationship with the peak area score in the range of 0.0 398- 0.3 184mg/ml（ r = 0.9 993） . The average recovery rate was 97.42 % （RSD = 1.84 % ）. CONCLUS ION ： The present method is accurate and reliable,and it can be used for the quality control of granati cortex.

关 键 词：高效毛细管电泳法 石榴皮 鞣花酸 含量 

分 类 号：R927.2[医药卫生—药学]