甲状腺球蛋白抗原决定簇基因的克隆及原核表达载体的构建  

The cloning of thyroglobulin epitopes gene and construction of its prokaryotic expression vector

作　　者：冯洁[1] 吕枚[1] 李宁[1] 方佩华[1] 杨立平[1] 崔景秋[1] 王少艳[1] 张红艳[1] 

机构地区：[1]天津医科大学总医院核医学科,天津300052

出　　处：《天津医科大学学报》2005年第4期550-553,共4页Journal of Tianjin Medical University

摘　　要：目的:扩增甲状腺球蛋白抗原决定簇基因,构建可转化入大肠杆菌的重组表达载体。方法:用TRIzol一步法从甲状腺组织中提取总RNA,RT-PCR逆转录出cDNA,PCR扩增出目的基因,与pET102/D-TOPO载体进行拓扑克隆,对重组质粒进行PCR、酶切及测序鉴定。结果:甲状腺球蛋白抗原决定簇基因及其重组表达载体经鉴定准确无误。结论:成功构建了带有目的基因的原核表达载体。Objective： The recombined the vector that can transfer thyroglobulin（Tg） epitopes gene into E.coli was constructed. Methods： Total RNAs were extracted from the human thyroid tissue of patient with Graves＇ disease（GD） by TRIzol reagent. The Tg epitopes gene was amplified by RT-PCR. The target gene was inserted into the expressive vectors of pET102/D-TOPO and then identified by PCR, restriction endonuclease and sequencing. Results： The recombined vector with Tg epitopes gene was correct by identification . Conclusion： The prokaryotic expression vector with target gene was constructed successfully.

关 键 词：甲状腺球蛋白 抗原决定簇基因 原核表达载体 

分 类 号：Q451[生物学—生理学] Q78