体外培养人胎盘滋养层细胞内吞丙型肝炎病毒过程的电镜观察  

HCV infection process in the in vitro human cytotrophoblasts under transmission electron microscope

作　　者：聂青和[1] 张亚飞[2] 罗新栋[2] 黄晓峰[3] 程勇前[2] 邵彬[2] 

机构地区：[1]第四军医大学唐都医院全军感染病诊疗中心710038 [2]第四军医大学唐都医院全军感染病诊疗中心 [3]第四军医大学电子显微镜室

出　　处：《中华传染病杂志》2005年第6期364-367,共4页Chinese Journal of Infectious Diseases

基　　金：国家自然科学基金资助项目(39970767)

摘　　要：目的探讨丙型肝炎病毒(HCV)感染体外分离培养人胎盘滋养层细胞的可能机制,观察 HCV 感染滋养层细胞后在细胞内的具体定位。方法采用胰蛋白酶消化法及 Percoll 密度梯度分离法分离培养人胎盘组织中滋养层细胞后,以 HCV RNA 阳性血清对滋养层细胞进行体外感染实验,不同时间终止实验.制备电镜标本,透射电镜观察。结果 HCV 感染滋养层细胞的过程中有吞噬小泡形成,形态学上符合被覆小凹的特点;感染后 HCV 病毒样颗粒多定位于粗面内质网附近。结论 HCV 可以感染滋养层细胞.其感染过程可能是被覆小凹介导的内吞过程。本研究提供了 HCV体外感染人胎盘滋养层细胞具体方式的形态学资料,对 HCV 宫内传播机制的进一步研究奠定了实验基础。Objective In order to in vitro investigate the possible mechanism of HCV infecting the human cytotrophoblasts cultured in vitro, and to observe the concrete location of HCV after infection. Methods Human placentae were digested with trypsin and centrifuged with percoll density gradient to obtain cytotrophoblasts. The eytotrophoblasts was incubated in DMEM-HG medium with HCV-RNA-positive serum. Stop the infection at different phases and prepare specimen for transmission electron microscope（TEM） examination. Results With TEM, an endocytic vesicle surrounding a HCV-like particle was investigated, which accorded with the morphologic characteristics of clathrincoated pits. HCV-like particles mainly located near rough endoplasmic reticulum, with clear envelope, either round-shaped or oval-shaped. Conclusions Cytotrophoblasts could be infected by HCV, and the process of HCV infection should be internalization via clathrin-coated pits. This study provides morphological evidences to identify that the cytotrophoblasts could be infected by HCV, which is the base of further study on the mechanism of HCV vertical transmission.

关 键 词：滋养层 肝炎病毒 疾病传播 垂直 显微镜检查 电子 透射 被覆小凹 

分 类 号：R512.63[医药卫生—内科学]