套式聚合酶链反应检测各孕期孕妇巨细胞病毒感染  

A study on cytomegalovirus Infection in pregnant women by nested polymerase chain reaction

作　　者：尚世强[1] 洪文澜[1] 余钟声[1] 潘存梅[1] 俞锡林[1] 郑兴宝[1] 石一复[1] 顾佩宝[1] 严宝瑾[1] 潘永观[1] 

机构地区：[1]浙江医科大学儿童医院,浙江医科大学妇产科医院

出　　处：《现代妇产科进展》1996年第2期119-122,共4页Progress in Obstetrics and Gynecology

基　　金：浙江省自然科学基金及浙江省卫生厅科研基金

摘　　要：目的评价套式聚合酶链反应（ＮＴ－ＰＣＲ）加限制酶切分析在各孕期人类巨细胞病毒（ｈＣＭＶ）感染检测中的应用。方法ｈＣＭＶ检测采用病毒分离、血清学试验、ＮＴ－ＰＣＲ加限制内切酶谱分析。结果３６７例孕妇中孕早、中、晚期ｈＣＭＶ阳性检出率分别为８．６％、１．６％及７．０％。经ＮＴ－ＰＣＲ检测，１８例ｈＣＭＶＤＮＡ阳性，阳性率为４．９％，而病毒分离，特异性ＩｇＭ及ＩｇＡ阳性率分别为３．０％、１．１％及２．２％。ＮＴ－ＰＣＲ阳性检出率高于病毒分离等其它方法（Ｐ＜０．０２５），与ＰＣＲ法比较，病毒分离特异性为１００％，敏感性为６１．１％，近３０％ｈＣＭＶ感染的孕妇ＰＣＲＤＮＡ阳性，表现为ＤＮＡ血症。结论ＮＴ－ＰＣＲ能早期检出孕妇ｈＣＭＶＤＮＡ，对孕妇ｈＣＭＶ感染的监控有重要意义。To evaluate the applicability of nested polymerase chain reaction (NTPCR) and restrictive endonuclease analyses in the diagnosis of human cytomegalovirus (hCMV) in pregnant women of different trimesters.Methods Diagnostic methods involved were virus isolation, serological tests. NTPCR and restrictive endonucleases analyses.Results Among 367 pregnant women.the infectious rate of the first. the second and the third trimester of gestation was 8. 6%,1. 60% and 7. 0%, respectively. The positive rate of NT-PCR was 4. 9 % (18/367),which was significantly higher than that of virus isloation ( 3. 0%), specific IgM ( 1. 1 % ) and specific IgA ( 2. 2% ), ( P< 0. 025). The specificity and the sensitivity of virus isolation were 100 % and 61. 1% ,when NT-PCR was used as a referance method. Nearly 30%cases of hCMV infection were positive by NT-PCR alone.Conclusion NT-PCR is a powerful tool for early detection of hCMV DNA in blood samples, which has an important significance in monitoring hCMV infection in pregnant women.

关 键 词：套式 聚合酶链反应 巨细胞病毒 感染 妊娠 

分 类 号：R714.251[医药卫生—妇产科学]