阻抑动力学光度法测定痕量尿素新方法研究  

Study on the Determination of Trace Urea by Inhibition Kinetic Spectrophotometry

作　　者：张振新[1] 孙登明[1] 朱庆仁[1] 杨缓凤 

机构地区：[1]淮北煤炭师范学院化学系,安徽淮北235000

出　　处：《淮北煤炭师范学院学报（自然科学版）》2005年第4期38-40,共3页Journal of Huaibei Coal Industry Teachers College(Natural Science edition)

基　　金：安徽省教育厅自然科学基金项目(2004KJ320);淮北市科技计划项目基金资助课题(040145)

摘　　要：研究了在盐酸介质中,微量尿素对亚硝酸根催化过氧化氢氧化亚甲基蓝褪色的阻抑作用,通过测量660nm处吸光度的变化,建立了测定痕量尿素的阻抑动力学光度分析的新方法.在最佳实验条件下,测定尿素的线性范围为0.1～2.0μg/25mL,检出限5.4×10-10g/mL.此方法用于废水中痕量尿素的测定,结果满意.A new catalytic inhibition kinetic spectrophotometric method for the determination of micro amounts of urea has been developed. The method is based on the inhibition effect of urea on the nitrite catalyzed oxidation of methylene blue by hydrogen peroxide in dilute hydrochloric acid medium. The absorbance is measured at 660 nm. Under the optimum conditions, the linear range of determination is 0. 10 - 2.0 μg/25mL. The detection limit is 5.4 ×10^-0 g/mL. The method is applied to the determination of urea in waste water with satisfactory results.

关 键 词：尿素 动力学光度法 阻抑反应 亚甲基蓝 

分 类 号：X786[环境科学与工程—环境工程]