NT_3基因修饰施万细胞促进移植的神经干细胞在损伤脊髓内分化为神经元样细胞  

NT-3 GENETICALLY MODIFIED SCHWANN CELLS PROMOTE GRAFTED NEURAL STEM CELLS DIFFERENTIATE INTO NEURON-LIKE CELLS IN THE INJURED SPINAL CORD

作　　者：郭家松[1] 曾园山[1] 李海标[1] 黄文林[2] 

机构地区：[1]中山大学中山医学院组织学胚胎学教研室神经科学研究室,第一军医大学分校广州510315 [2]中山大学中山医学院肿瘤防治中心,广州510080

出　　处：《解剖学报》2005年第6期577-581,共5页Acta Anatomica Sinica

基　　金：国家重点基础发展规划资助项目(19990540009);国家自然科学基金资助项目(30270700);广东省社会发展攻关资助项目(2003C33808)

摘　　要：目的探讨神经营养素-3(NT-3)基因修饰施万细胞(SCs)对植入全横断性脊髓损伤处的神经干细胞(NSCs)分化为神经元样细胞的影响。方法将NSCs单独移植或与NT-3基因修饰SCs、LacZ基因修饰SCs和SCs联合移植到全横断性脊髓损伤处,67 d后用免疫组织化学方法观测NSCs的分化,并计算其中神经元样细胞的分化率。结果NSCs在损伤脊髓内可分化为神经元样细胞和神经胶质样细胞,与未基因修饰的SCs的作用相比,NT-3基因修饰的SCs能更有效地提高NSCs向神经元样细胞的分化率,而LacZ基因修饰的SCs与未修饰SCs没有明显区别。结论NT-3基因修饰SCs能促进NSCs在损伤脊髓内向神经元样细胞分化。Objective To explore the effects of Neurotrophin-3 （NT-3） genetically modified Schwann cells （SCs） on grafted neural stem cell（NSC） differentiation into the neuron-like cells in the transected spinal cord. Methods The NSCs were single-transplanted or co-engrafted with NT-3 modified SCs,report gene LacZ modified SCs,and unmodified SCs respectively into the transected spinal cord 67d later, the differentiation of NSCs was studied by immunohistochemisty, and the percentage of neuronlike cells was calculated. Results NSCs could differentiate into the neuroglial-like cells and neuron-like cells in the injured spinal cord. Compared to the unmodified SCs, NT-3 modified SCs could more efficiently promote NSCs differentiate into the neuronlike cells .The effect of LacZ modified SCs was the same to the unmodified SCs. Conclusion NT-3 modified SCs could promote NSCs differentiate into the neuron-like cells in the injured spinal cord.

关 键 词：神经干细胞 神经营养素-3 基因修饰施万细胞 分化 神经元样细胞 脊髓损伤 免疫组织化学 大鼠 

分 类 号：R338.21[医药卫生—人体生理学]