VR1受体在大鼠食管传入纤维的表达  

THE EXPRESSION OF THE VANILLOID RECEPTOR SUBTYPE 1 IN AFFERENT FIBERS OF THE RAT ESOPHAGUS

作　　者：王昭金[1] 王延清 

机构地区：[1]泰山医学院解剖学教研室,泰安271000 [2]山东省泰山慢性病医院,泰安271000

出　　处：《解剖学报》2005年第6期675-678,共4页Acta Anatomica Sinica

基　　金：山东省中青年学术骨干和学科带头人基金资助项目

摘　　要：目的研究辣椒素(capsaicin)受体VR1在大鼠食管传入神经纤维的表达,纤维的形态特征及分布。方法应用激光共聚焦显微镜结合免疫组织化学双标技术。结果VRl阳性(VR1-IR)纤维和终末主要分布于食管的黏膜层、黏膜下层、平滑肌以及血管周围,其形态多为一些含有小棘及串珠样结构的细纤维。有(92.3±3.7)%VR1-IR纤维与代表脊神经节源性传入纤维的神经肽CGRP共存。VR1在脊神经节和结状神经节中的表达类似,为中小型神经元。脊神经节中有(41.5±4.5)%VRl-IR神经元含有CGRP和(67.9±3.2)%CGRP-IR神经元表达VR1。CGRP-IR细胞在结状神经节较少,仅有(4.7±1.4)%VR1-IR神经元含有CGRP。结论大鼠食管壁内的脊神经源性感觉纤维表达VR1受体。Objective To study the expression of the capsaicin receptor, vanilloid receptor subtype 1 （VR1） immunoreactivity, their morphology and distribution in afferent fibers of rat esophagus. Methods Laser scanning confocal microscope combined with immunohistochemical double labeling methods were used. Results VRl-like immunoreactivity was observed on nerve fibers and terminalis within mucosa, submucosa, muscle layer and surrounding blood vessel throughout esophagus. Their profiles were fine fibers with some spiny, possessing varicose-like swellings along their lengths. About（92.3 ± 3.7） % VRI positive fibers co-localized with CGRP immunoreactivity, which representing large majority of afferent fibers in the esophagus was extrinsic in origin with cell bodies located in dorsal root ganglia. In dorsal root ganglion, VR1 was expressed in small-and middle-sized cell bodies. About （41.5 ± 4.5 ） % VRl-immunoreactive neurons co-stained with CGRP and （ 67.9 ± 3.2） % CGRP positive neurons co-localized with VR1. In nodose ganglion, the expression of VR1 was similar with dorsal root ganglion, but CGRP immunoreactive neurons very few. Only（4.7 ± 1.4）% VR1 positive neurons co-stained with CGRP. Conclusion These results suggest that VR1 is expressed in afferent fibers in the wall of the rat esophagus which is in origin in dorsal root ganglion.

关 键 词：传入纤维 VR1受体 降钙素基因相关肽 食管 免疫组织化学 激光共聚焦显微镜 大鼠 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]