幼年与成年兔椎间盘髓核细胞的形态学差异及其意义  

COMPARISON OF MORPHOLOGIC CHARACTERISTICS OF THE NUCLEUS PULPOSUS CELLS FROM YOUNG AND ADULT RABBIT INTERVERTEBRAL DISC

作　　者：刘小云[1] 李康华[1] 宋建华[2] 郭广松[3] 梁昌镛[2] 王锡阳[1] 陈新文[2] 

机构地区：[1]中南大学湘雅医院骨科,长沙410008 [2]中国科学院武汉病毒研究所病毒学国家重点实验室,武汉430071 [3]武汉大学中南医院病理科,武汉430071

出　　处：《解剖学报》2005年第6期679-681,共3页Acta Anatomica Sinica

基　　金：国家973资助项目(2003CB114202)

摘　　要：目的探讨幼年与成年兔髓核细胞的形态学差异。方法利用激光共聚焦显微镜和透射电镜,对幼年和成年兔的髓核组织进行组织细胞水平和超微结构的观察。结果幼年兔的髓核细胞呈圆形,簇状分布,其直径为(266±167)μm,细胞簇的密度为每个高倍(×40)视野(14.9±4.3)个,细胞内含有大量空泡状的包含体。成年兔的髓核细胞呈圆形或类圆形,细胞簇小或呈单个散在分布,细胞簇的直径为(94±42)μm,细胞簇密度为每个高倍(×40)视野(8.0±2.4)个细胞,其中包含体数目少且不含大的包含体。结论幼年与成年兔的髓核细胞具有明显的形态结构差异。椎间盘成年期发生的这种形态结构上的变化可能是随年龄增加而椎间盘生物学功能逐渐降低的原因之一。Objective To compare the cellular characteristics of the nucleus pulposus （NP） ceils of young and adult rabbits. Methods The structure and arrangement of the NP cells from young and adult rabbits were investigated on both tissue and uhrastructure level by confocal laser scanning microscope and transmission electron microscope. Results The NP cells from the young rabbit discs, which were round and contained numerous large inclusion bodies, often formed clusters with a diameter of （266 ± 167） μm and a density of （8.0 ± 2.4） per high power（ × 40） ; The NP cells from the adult rabbit discs were round or oval shape and had no large size inclusion bodies, formed less clusters or were singly diffusedly distributed, with the diameter of clusters to be （94 ±42） μm （ single cell excluded） and density to be （ 14.9 ± 4.3） per high power （ × 40）. Conclusion There were remarkable morphological differences in the cell architecture of nuclei pulpesi between the young and adult rebbits. These features of the aging and degenerating disc contribute to decreased biological function.

关 键 词：椎间盘 髓核细胞 形态学 激光共聚焦显微镜 透射电镜 兔 

分 类 号：R681.5[医药卫生—骨科学]