提高大白菜小孢子植株获得率的研究  

Studies on Enhancing of Plantlet Regeneration in Isolated-microspore Culture of Chinese Cabbage

作　　者：韩阳[1] 叶雪凌[2] 冯辉[2] 

机构地区：[1]辽宁大学生命科学系,沈阳110036 [2]沈阳农业大学园艺学院,沈阳110161

出　　处：《园艺学报》2005年第6期1092-1094,共3页Acta Horticulturae Sinica

基　　金：辽宁省自然科学基金资助项目(20022041)

摘　　要：大白菜小孢子胚发生过程中胚胎发育不同步,以子叶形胚数量最多。发育较好的胚状体成苗率较高,子叶形胚和萌发胚成苗率分别为20%和90.5%;较干燥的培养基更利于小孢子胚成苗,最适的琼脂含量是1.2%。培养基中添加200 mg.L-1活性炭可以大幅度提高小孢子胚成苗率,及时地将成熟的子叶形胚转移至再生培养基上,对提高植株获得率至关重要。During the microspore embryogenesis of Chinese Cabbage, the embryo development was not synchronism and cotyledonary embryos was the highest （80%）. The better embryos develop, the yield of higher plantlet regeneration rate is. And the regenerated plant rate of cotyledonary embryos and germinated embryos were 20% and 90. 5% respectively. Dry culture medium was favor of regenerating plantlet, optimum agar content was 1.2%. Culture medium supplemented with activated charcoal 200 mg · L^-1 could evidently raise regenerated plant rate, which was as 2. 225 times as control （without activated charcoal）. Transferring mature cotyledonary embryos to the regenerating culture medium in time was essential to increasing plantlet regeneration rate.

关 键 词：大白菜 游离小孢子培养 胚状体 植株再生率 

分 类 号：S634.1[农业科学—蔬菜学] S603.6[农业科学—园艺学]