甜瓜属人工异源四倍体早期基因组变化的初步研究  

Preliminary Studies on Early Genomic Changes of a Synthetic Allotetraploid in Cucumis

作　　者：陈龙正[1] 娄群峰[1] Joseph N.Wolukau 陈劲枫[1] 耿红[1] 罗向东[1] 

机构地区：[1]南京农业大学园艺学院作物遗传与种质创新国家重点实验室,南京210095

出　　处：《园艺学报》2005年第6期1105-1107,共3页Acta Horticulturae Sinica

基　　金：国家高技术研究发展计划专项经费资助项目(2004AA241120);国家自然科学基金资助项目(30470120);博士点基金项目(20050307009)

摘　　要：利用AFLP技术对甜瓜属人工异源四倍体(Cucum is×hytivusChen and K irkbride,2n=38)不同自交世代及二倍体亲本DNA进行分析,结果表明多倍体基因组在形成早期发生了广泛的变化,变化位点所占比例高达22.2%。基因组序列变化始于F1代,最高变化率出现在S2代。基因组变化主要表现为部分亲本片段丢失、新片段产生以及后代对亲本某些位点的跳跃式继承。还观察到部分双亲差异性条带呈现出偏父性遗传的特点。AFLP analysis was carried out in amplification of the DNAs from different generations of the synthetic allotetraploid （ Cucumis x hytivus, Chen and Kirkbride, 2n = 38 ） , the interspecific F1, and its diploid parents. The results indicated that extensive genomic changes occurred in the synthetic allotetraploid, and the variation rate was as high as 22.2%. We found the genomic changes started in the F1 and the highest variation rate observed in S2. Most genomic changes involved the loss of parental fragments, formation of novel fragments, with some fragments disappearing in one generation and reappearing in others. In addition, it was observed that some parent-specific bands were inherited preferentially from the male parent.

关 键 词：甜瓜属 异源四倍俸 AFLP分析 基因组序列变异 

分 类 号：S652[农业科学—果树学]