大鼠脑海马CA1区神经元诱发放电过程中催产素与阿片受体的参与和调制作用(英文)  

Involvement and modulation effects of oxytocin and opioid receptor on evoked discharge of hippocampal CA1 neurons in rats

作　　者：陈静芳[1] 胡祁生[2] 胡胜娣[2] 邹祖玉[3] 王海梅[2] 

机构地区：[1]广东医学院生理教研室,广东省湛江市524023 [2]武汉大学医学院生理系,湖北省武汉市430071 [3]武汉大学医学院病理学教研室,湖北省武汉市430071

出　　处：《中国临床康复》2005年第45期158-160,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:中枢内催产素可作为一种神经递质或调质参与学习记忆、性行为、痛觉调节以及阿片耐受与依赖,海马内催产素能系统与阿片系统具有一定的相互作用。目的:探讨侧脑室注射催产素对大鼠脑左背侧海马CA1区神经元诱发放电的影响以及催产素与阿片受体之间可能的相互作用。设计:随机对照实验。单位:广东医学院生理教研室,武汉大学医学院生理系,武汉大学医学院病理学教研室。材料:实验于2002-09/2003-09在武汉大学医学院生理系完成,选用雄性SD大鼠36只。随机分为6组,生理盐水对照组、催产素处理组(0.2,2,20mg/L)、催产素受体拮抗剂+催产素(2mg/L)组、纳洛酮+催产素(2mg/L)组,每组6只。方法:脑海马CA1区细胞外记录采用玻璃微电极记录:用微电极推进器将电极插入脑海马CA1区。神经元放电经微电极放大器放大后,显示在示波器上进行观察。记录到神经元放电后,经双极不锈钢电极每隔5min刺激坐骨神经1次,共刺激50min10次。催产素0.2,2,20mg/L处理组通过侧脑室给药管匀速而缓慢注入催产素0.2,2,20mg/L5μL。催产素受体拮抗剂+催产素(2mg/L)组:侧脑室预先注射80mg/L催产素受体拮抗剂2.5μL,然后再注射2mg/L催产素2.5μL。纳洛酮+催产素(2mg/L)组:侧脑室预先注射400mg/L纳洛酮2.5μL,然后再注射2mg/L催产素2.5μL。以放电频率变化率为观察指标,检测不同剂量催产素对海马CA1区神经元诱发放电的影响及催产素受体拮抗剂、纳洛酮对催产素的作用。主要观察指标:各组大鼠刺激前后脑海马CA1区神经元诱发放电频率的变化率。结果:36只大鼠均进入结果分析。①侧脑室分别注射0.2,2,20mg/L催产素5μL使海马CA1神经元诱发放电频率明显降低,剂量依赖关系明显。②催产素(2mg/L)对CA1神经元诱发放电的抑制作用可被侧脑室预先注射催产素受体拮抗剂(80mg/L,2.5μL)所阻断。③侧脑室注射纳洛酮(40BACKGROUND： Central oxytocin （OT） may be a neurotransmitter or neuromodulator and play an important role in learning and memory, sexual behaviour, pain modulation and opiate tolerance and dependence. To research the interactions between oxytocinergic and opioidergic system in hippocampus has some significance. OBJECTIVE： To investigate the effects of OT administered intracere-broventricularly on evoked discharge of left dorsal hippocampal CA1 neurons in rats and the possible interactions between oxytocinergic and opioidergic system. DESIGN： A randomised controlled study. SETTING： Department of Physiology of Guangdong Medical College; Department of Physiology and Pathology of Medical College of Wuhan University. MATERIALS： The study was conducted in the Physiology Department of Medical College of Wuhan University from September 2002 to September 2003. A total of 36 male Spragne-Dawley rats were selected and randomly divided into six groups： control （NS）, OT groups （0.2 mg/L, 2 mg/L and 20 mg/L）, [d（CH2）5-OVT]＋OT （2 rag/L）, naloxone＋OT （2 mg/L）, with 6 rats in each group. METHODS： Single-unit recording was performed with glass microelectrode. The glass microelectrode was inserted by a micromanipulator into hippocampal CA1. The electrical activity was amplified by a microelectrode amplifier and then recorded by the biological experimental system, monitored at the same time with oscilloscope. When recording the neural discharge, electrical stimulation of the sciatic nerves was performed once 5 minutes through a double stainless electrode. 5μL oxytocin in dosage of 0.2, 2 and 20 mg/L were injected slowly into lateral ventricle via microlitre syringe. [d（CH2）5-OVT]＋OT （2mg/L） group： 2.5μL [d（CH2）5-OVT] （80 mg/L） was injected into lateral ventricle and then 2.5 μL oxytocin （2 mg/L）. Naloxone＋OT （2 mg/L） group： 2.5 μL naloxone （400 mg/L） was injected into lateral ventricle and then 2.5 μL oxytocin （2 mg/L）. According to frequ

关 键 词：学习与记忆 海马 受体 催产素 纳洛酮 

分 类 号：R347.531[医药卫生—基础医学]