灯盏花素注射液对沙土鼠脑缺血-再灌注后能量代谢及脑水肿的影响(英文)  

Effects of breviscapine parenteral solution on energy metabolism and cerebral edema after cerebral ischemia-reperfusion in gerbils

作　　者：王建国[1] 陈群[2] 曾因明[2] 

机构地区：[1]济宁医学院附属医院麻醉科,山东省济宁市272000 [2]徐州医学院附属医院江苏省麻醉学重点实验室,江苏省徐州市221002

出　　处：《中国临床康复》2005年第45期177-179,共3页Chinese Journal of Clinical Rehabilitation

基　　金：江苏省青年科技基金(BQ98009);江苏省麻醉学重点实验室开放课题基金(K9844)~~

摘　　要：背景:脑缺血-再灌注后能量代谢障碍和脑水肿是脑缺血-再灌注损伤重要因素之一。中药灯盏花素注射液(其有效成分为黄芩素甙)可以防止脑缺血-再灌注诱发的蛋白激酶C的激活、减轻钙超载,且可减小缺血梗死灶的体积,从而减轻脑缺血-再灌注损伤。可灯盏花素注射液对脑缺血-再灌注后能量代谢和脑水肿有何影响?目的:观察灯盏花素注射液对沙土鼠脑缺血-再灌注后能量代谢和脑水肿的影响。设计:随机对照实验。单位:济宁医学院附属医院及徐州医学院附属医院江苏省麻醉学重点实验室。材料:实验于1999-02/08在江苏省麻醉学重点实验室完成。选用雄性沙土鼠72只。方法:将实验动物随机分成3组,即假手术组、常温对照组和灯盏花素组,假手术组8只,常温对照组和灯盏花素组每组各32只。根据再灌注时间将常温对照组和灯盏花素组分4个亚组,即缺血末组、再灌注10min组、再灌注30min组和再灌注60min组。每亚组8只动物。常温对照组和灯盏花素组制备前脑缺血-再灌注损伤模型,脑缺血时间为10min。假手术组仅游离双侧颈总动脉但不予阻断。灯盏花素组于缺血前15min给予灯盏花素注射液90mg/kg腹腔注射,假手术组和常温对照组则给予等量的生理盐水。脑水的测定采用干湿重法,应用高效液相及紫外检测仪测定海马三磷酸腺苷,二磷酸腺苷,磷酸腺苷的含量。主要观察指标:①实验动物海马三磷酸腺苷、二磷酸腺苷、磷酸腺苷的含量。②实验动物脑皮质水含量。结果:纳入实验的动物为72只,均进入结果分析,无实验动物脱失。①实验动物海马三磷酸腺苷、二磷酸腺苷、磷酸腺苷的含量:常温对照组在缺血末、再灌注10min、再灌注30min、再灌注60min时,海马组织三磷酸腺苷和腺苷酸池含量明显降低,三磷酸腺苷含量分别为假手术组的68%,56%,49%和50%(P均<0.01),腺苷酸池含量为假BACKGROUND： The disorder of energy metabolism and cerebral edema after cerebral ischemia-reperfusion are the important factors to inducecerebral isehemia-reperfusion injury. The Chinese herb, breviscapine, whose effective component is scutellarin, can prevent the activation of protein kinase C evoked by ischemia-reperfusion, relieve calcic overload and reduce the volume of ischemia infarcted focus volume, and then alleviate cerebral ischemia-reperfusion injury. But what are the influences of breviscapine on energy metabolism and cerebral edema after cerebral ischemia-reperfusion？ OBJECTIVE： To observe the effects of breviscapine parenteral solution on energy metabolism and cerebral edema after cerebral ischemia-reperfusion in gerbils. DESIGN： A randomized control trial. SETTING： Affiliated Hospital of Jining Medical College, Jiangsu Provincial Key Laboratory of Anesthesiology, Affiliated Hospital of Xuzhou Medical College. MATERIALS： The experiment was carried out in the Jiangsu Provincial Key Laboratory of Anesthesiology Between February and August 1999. Seventy-two male gerbils were used. METHODS： The gerbils were randomly divided into sham-operated group （n=8）, normothermia control group （n=32） and breviscapine group （n=32）. According to the reperfused time, the normothermia control group and breviscapine group were divided into 4 subgroups with 8 gerbils in each subgroup： 0, 10, 30 and 60-minute reperfusion groups. The gerbils in the normothermia control group and breviscapine group were made into models of forebrain ischemia reperfusion, treated with ischemia for 10 minutes. In the shamperated group, only bilateral common carotid arteries were freed but not occluded. In the breviseapine group, the gerbils were given intraperitoneal injection of breviscapine parenteral solution （90 mg/kg） at 15 minutes before ischemia. The gerbils in the sham-operated group and normothermia control group were treated with saline of the same volume. The brain water was determined by dryi

关 键 词：脑缺血 灯盏细辛 腺苷三磷酸 脑水肿 

分 类 号：R255.2[医药卫生—中医内科学]