草问荆总生物碱对大鼠脑内单胺类神经递质含量的影响(英文)  

Effect of total alkaloid of Equisetum pratense on the contents of monoamine neurotransmitters in brain of rats

作　　者：季宇彬[1] 高世勇[1] 

机构地区：[1]哈尔滨商业大学药物研究所博士后科研工作站,黑龙江省哈尔滨市150076

出　　处：《中国临床康复》2005年第45期185-187,共3页Chinese Journal of Clinical Rehabilitation

基　　金：黑龙江省自然科学基金项目(D01-05);黑龙江省科技厅重大攻关项目(GB04C404);黑龙江省专利补贴基金;黑龙江省专科产业化项目~~

摘　　要：背景:从天然药物中寻求安全有效的镇静安定类药物是逐年增加的神经精神类疾病治疗的需要。前期实验发现草问荆总生物碱具有良好的镇静安定作用。目的:分析草问荆总生物碱对中枢神经系统的抑制作用。设计:以实验动物为观察对象的随机对照实验。单位:哈尔滨商业大学药物研究所博士后科研工作站。材料:实验于2002-03/2003-01在黑龙江中医药大学药理研究室完成。实验药物为草问荆总生物碱;实验动物选用Wister大鼠24只。方法:24只大鼠随机分为3组,分别为草问荆总生物碱组(60mg/kg)、利血平组(30mg/kg)、生理盐水组(同体积),腹腔一次性给药上述剂量药物后,采用高效液相色谱-电化学检测法及紫外线检测法测定大鼠脑内单胺类神经递质含量。主要观察指标:大鼠纹状体和脑边缘区单胺类递质去甲肾上腺素、肾上腺素、多巴胺和5-羟色胺及其代谢产物3,4-二羟苯乙二醇、5-羟吲哚乙酸、高香草酸的含量。结果:实验前和实验后动物数均为每组8只,无异常或死亡现象。草问荆总生物碱对大鼠纹状体的单胺类神经递质的含量具显著的降低作用,同时升高纹状体单胺递质中性及酸性代谢产物的含量。对大鼠前脑边缘区的单胺类递质具有显著的降低作用,同时显著升高边缘区单胺代谢物5-羟吲哚乙酸,高香草酸的含量,但对于3,4-二羟苯乙二醇的升高作用无统计学意义(P>0.05)。结论:草问荆总生物碱的镇静、安定作用与中枢的单胺类神经递质的含量下降有关。草问荆总生物碱具有与利血平相似的单胺排空作用,是草问荆总生物碱对中枢神经系统镇静安定作用的作用机制。BACKGROUND： It is needed in the treatment of yearly increased nervous and mental diseases to seek safe and effective sedatives and tranquilizers from natural drugs. It is found in the previous experiments that the total alkaloid of equisetum pratense （TAEP） has the satisfactory sedative and tranquitrzing actions. OBJECTIVE： To analyze the inhibitory effect of TAEP on the central nervous system. DESIGN： A randomized control study taking experiment animals as the observing objects. SETTING： The Post-doctor Scientific Research Station, Institute of Materia Medica, Harbin Shangye University. PARTICIPANTS： The experiment was carried out in the Department of Pharmacology, Heilongjiang University of Traditional Chinese Medicine between March 2002 and January 2003. The drug was TAEP, and 24 Wistar rats were used. METHODS.： Twenty-four rats were randomly divided into 3 groups： TAEP group （60 mg/kg）, reserpine group （30 mg/kg） and saline group （the same volume）, all the rats were given intiTaperitoneal injections of the above drugs in corresponding dosages, and then the contents of monoamine neurotransmitters in brain were determined by high performance liquid chromatography （HPLC）-electrochemistry （HPLC-EC） and HPLC-ultraviolet （HPLC-UV） respectively. MAIN OUTCOME MEASURES： The contents of monoamine neurotranmitters of norepinephrine, adrenalin, dopamine and 5-serotonin （5-HA） and its metabolites of DHPR, 5-hydroxyindoleacetic acid （5-HIAA） and homovanillic acid in the striatun and marginal area of brain in rats were observed. RESULTS： There were 8 rats in each group both before and after the experiment without abnormalities or death. TAEP significantly lowered the contents of monoamine neurotranmitter but increased the contents of neutral and acidic metabolites of monoamine neurotranmitters in the striatum of rats. TAEP significantly decreased the monoamine neurotranmitters and significantly elevated the contents of monoamine metabolites of 5-HIAA and ho

关 键 词：生物碱类 神经抑制 生物源单胺类 神经递质 

分 类 号：R917.78[医药卫生—药物分析学]