上海地区胰升糖素样肽1受体基因多态性与2型糖尿病相关  

Association of single nucleotide polymorphism in glucagon-like peptide-1 receptor gene with type 2 diabetes in Shanghai

作　　者：郑昇[1] 骆天红[1] 赵萸[1] 李果[1] 刘珉[1] 顾燕云[1] 张宏利[1] 刘优萍[1] 罗敏[1] 

机构地区：[1]上海第二医科大学附属瑞金医院内分泌代谢病科,上海市内分泌代谢病临床中心,上海市内分泌代谢病研究所,200025

出　　处：《中华内分泌代谢杂志》2005年第6期511-513,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：国家科技攻关基金资助项目(2002BA711A05);国家自然科学基金资助项目(30100084);全国博士论文作者专项资金资助项目(200360);上海市启明星计划基金资助项目(03QC14040);国家自然科学基金资助项目(30571019)

摘　　要：目的研究胰升糖素样肽1受体(GLP1R)基因的单核苷酸多态性(SNP)与上海地区汉族人群2型糖尿病的相关性。方法选取上海地区无亲缘关系的2型糖尿病患者360例及正常对照313名,其中糖尿病患者分为肥胖组192例(BMI>28kg/m2,且仅用口服降糖药治疗)及非肥胖组168例(BMI<25kg/m2,且用胰岛素治疗),采用等位基因特异的实时PCR,对GLP1R基因位点rs2268657进行基因分型,并通过相关分析,研究该位点与2型糖尿病的相关性。结果GLP1R基因rs2268657位点AA、AG、GG基因型频率在对照人群中分别为0.086,0.447,0.446;在非肥胖糖尿病组中分别为0.155,0.375,0.470,在肥胖糖尿病组中分别为0.109,0.500,0.391。在正常人基因型AA频率与非肥胖糖尿病组相比差异有统计学意义(OR=1.939,P<0.05),而与肥胖糖尿病组相比差异无统计学意义。结论GLP1R基因多态性位点rs2268657可能与胰岛素分泌不足为主的2型糖尿病有关。Objective To study the association of glucagon-like peptide-1 receptor （GLPIR） gene polymorphism with type 2 diabetes in Han population in Shanghai. Methods In the study, 360 type 2 diabetic patients and 313 normal control subjects were enrolled. Diabetic patients were further subdivided into insulintreated non-obese patients （BMI 〈 25, 168 subjects） and oral anti-diabetic agents-treated obese patients （BMI 〉 28, 192 subjects）. A single nucleotide polymorphism （SNP） rs 2268657 was genotyped in all the subjects enrolled in the study using allele-specific real-time PCR and its association with type 2 diabetes was examined. Results The frequencies of AA, AG, GG genotype in control group were 0. 086, 0.447, 0.466 respectively, 0. 155, 0. 375, 0. 470 in non-obese diabetic patient group respectively, and 0. 109, 0. 500, 0. 391 in obese diabetic patient group respectively. There was significant difference of the frequency of genotype AA between control group and non-obese diabetic patient group （ OR = 1. 939, P 〈 0.05 ）, and no significant difference existed between control group and obese diabetic patient group. Conclusion The SNP rs 2268657 of GLP1R gene is probably associated with type 2 diabetes characterized by insulin deficiency.

关 键 词：糖尿病 2型 胰岛素分泌 多态性 单核苷酸 

分 类 号：R587.1[医药卫生—内分泌] R681.02[医药卫生—内科学]