血清白蛋白与锌(Ⅱ)-精氨酸络合物相互作用的极谱法研究与应用  

Studies and Application of the Interaction of Protein with Zn(Ⅱ)-Arginine Complex by Using Polarography

作　　者：杨慧林[1] 罗登柏[1] 

机构地区：[1]中南民族大学化学与材料科学学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2005年第4期1-3,共3页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：湖北省自然科学基金资助项目(2005ABA001);中南民族大学自然科学基金重点资助项目(YZZ03003)

摘　　要：在pH9.1的乙醇胺-盐酸介质中,血清白蛋白与锌(Ⅱ)-精氨酸络合物反应作用生成一种在滴汞电极上电化学惰性的锌(Ⅱ)-血清白蛋白络合物,使锌(Ⅱ)-精氨酸络合物在-1.20V(vs.SCE)络合吸附波还原峰电流降低,峰电流降低值与2～28 mg/L范围内牛血清白蛋白(BSA)或1～29 mg/L范围内人血清白蛋白(HSA)呈线性关系;检出限分别为0.2和0.4 mg/L.应用该法测定了人血清样品中总蛋白含量.The interaction of protein with Zn（ Ⅱ ）-arginine complex in pH 9.1 buffer solution has been studied by single sweep polarography. Adding bovine serum albumin （BSA） / human serum albumin （HSA） into the Zn（ Ⅱ ）- arginine complex solution results in the formation of electrochemical non-active complex of Zn （ Ⅱ）-BSA / HSA, and causes the current decrease of the adsorption peak of Zn （ Ⅱ ）- arginine complex . The △ip is linearly proportional to the protein concentration from 2 to 28 mg/L for BSA and from 1 to 29 mg/L for HSA. The detection limit is 0.2 mg/L for BSA and 0.4 mg/L for HSA. The method has been applied to the determination of total protein content in samples of human serum.

关 键 词：血清白蛋白 单扫描极谱法 锌(Ⅱ)-精氨酸络合物 锌(Ⅱ)-血清白蛋白络合物 

分 类 号：O657.14[理学—分析化学]