Subcellular distribution and compartmentalization of arsenic in Pteris vittata L.  

作　　者：CHEN Tongbin YAN Xiulan LIAO Xiaoyong XIAO Xiyuan HUANG Zechun XIE Hua ZHAI Limei 

机构地区：[1]Center for Environmental Remediation, Institute of Geographic Sciencesand Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China

出　　处：《Chinese Science Bulletin》2005年第24期2843-2849,共7页

基　　金：supported by the National Science Foundation for Distinguished Young Scholar(Grant No.40325003);the.National Natural Science Foundation of China(Grant No.40232022);the National High-Tech R&D Program(No.2003AA645010).

摘　　要：The subcellular distribution of arsenic (As) in Pteris vittata L., an As-hyperaccumulator, was studied to de- termine As compartmentalization and to explore the mecha- nisms that confer As tolerance. When the plant was grown in a nutrient solution without additional As, most of the accu- mulated As was isolated to the cell wall. However, in plants growing in a nutrient solution containing 0.1 or 0.2 mmol/L As, approximately 78% of the total As accumulated within the pinna. The proportions of As accumulation in the cyto- plasmic supernatant fraction were 78% of that in the pinna and 61% of that in the plant. In either treatment group (0.1 or 0.2 mmol/L As), the fraction containing the lowest level of As was the organelle fraction. These results suggest that As accumulates in the pinna where it is primarily distributed in the cytoplasmic supernatant fraction. The role of As com- partmentalization may be intricately linked with As detoxi- fication in P. vittata L.The subcellular distribution of arsenic （As） in Pteris vittata L., an As-hyperaccumniator, was studied to determine As compartmentalization and to explore the mechanisms that confer As tolerance. When the plant was grown in a nutrient solution without additional As, most of the accumulated As was isolated to the cell wall. However, in plants growing in a nutrient solution containing 0.1 or 0.2 mmol/L As, approximately 78% of the total As accumulated within the pinna. The proportions of As accumulation in the cyto- plasmic supernatant fraction were 78 % of that in the pinna and 61% of that in the plant. In either treatment group （0.1 or 0.2 mmol/L As）, the fraction containing the lowest level of As was the organelle fraction. These results suggest that As accumulates in the pinna where it is primarily distributed in the cytoplasmic supernatant fraction. The role of As compartmentalization may be intricately linked with As detoxification in P. vittata L.

关 键 词：砷 超量聚积植物 环境污染 亚细胞分布 凤尾蕨属 

分 类 号：X503.23[环境科学与工程—环境工程]