抗骨增生胶囊加葡立胶囊和基因治疗对家兔退变颈椎间盘IL-1β、TNF-α含量变化的影响  

Experimental study of the Anti-hyperosteogeny capsule(抗骨增生胶囊)combined with Glucosamine Hydrochloride capsule(葡立胶囊)and gene therapy on influence in the contents of IL1β,TNF-α of degenerated cervical intervertebral discs in rabbits

作　　者：刘福成[1] 赵晓勇[1] 王海涛[1] 苏江宁[1] 孙喜龙[1] 

机构地区：[1]河北省人民医院骨科,河北石家庄050051

出　　处：《中国骨伤》2005年第11期670-672,共3页China Journal of Orthopaedics and Traumatology

基　　金：河北省科学技术研究与发展计划项目(03276196D-50)

摘　　要：目的：研究抗细胞因子的中西药联合应用、基因治疗对兔退变颈椎间盘内细胞因子含量变化的影响。方法：选用35只4个月龄新西兰兔，体质量2～3kg，雌雄不分，并随机分为正常对照组、模型浅层组、模型全层组、药物治疗浅层组、药物治疗全层组、基因治疗浅层组、基因治疗全层组。建立家兔颈椎动力平衡失调模型，诱导颈椎间盘退变（正常对照组不作处理）。术后7个月，药物治疗组（浅层、全层）给予抗骨增生胶囊和葡立胶囊（剂量按体质量折算）灌胃，2次／d，连用1个月；基因治疗组则将带有转化生长因子β（TGF-β1）基因的重组质粒DNA注入C2—C5椎间盘中（每个椎间盘用量为20μl）。8个月后用双抗夹心ELISA法检测各组动物颈椎间盘中IL-1β、TNF-α抗体含量。结果：模型组与正常对照组比较，颈椎间盘中IL-1β、TNF-α含量均明显升高（P〈0．01）；中西药治疗和基因治疗组与模型组比较IL-1β、TNF-α含量明显降低（P〈0．05）。结论：退变颈椎间盘组织释放多种细胞因子和炎性介质，它们可加速颈椎间盘退变。中西药联合应用及基因治疗能对其含量起明显的抑制作用。Objective：To study the anticytokine Chinese herb combined with western drug and gene therapy on influence in the contents of IL-1β,TNF-α of degenerated cervical intervertebral discs in rabbits. Methods：Thirty-five four-month-old New Zealand rabbits （weight 2～3 kg） were randomly divided into seven groups： nomaal group, model group （shallow layer ）, model group （ whole layer ）, drug group （ shallow layer ）, drug group （whole layer）,gene group （shallow layer）,gene group （whole layer）. In all groups except for the nomaal group, the cervical dynamic unbalance model of rabbits were made to induce to intervertebral disc degeneration. Seven months after operation, the drug groups were fed with Anti-hyperosteogeny capsule and Glucosamine Hydrochloride capsule twice a day for 1 month. The gene groups were infused recombinant plasmid DNA of TGF-β1 in intervertebral discs of C2 - C5,20μl in each disc. After eight months,the contents of IL-1β and TNF-α of degenerated cervical intervertebral discs of every group were assayed with ELISA. Results：The contents of IL-1β and TNF-α in model group were raised,there was significantly difference as compared with control group （P〈0.05）. The contents of IL-1β and TNF-α in drug group and gene group were decreased, there was significantly difference as compared with model group （P 〈 0.05）. Conclusion： The degenerated intervertebral disc releases many kinds of cytokine and inflammatory facters to accelerate the degeneration. Antihyperosteogeny capsule combined with Glucosamine Hydrochloride capsule and gene therapy have inhibition of the contents of IL-1β and TNF-α in degenerated cervical intervertebral disc.

关 键 词：椎间盘 抗骨增生胶囊 葡立胶囊(盐酸氨基葡萄糖) 转化生长因子Β 细胞因子 

分 类 号：R631.205[医药卫生—外科学] R681.5[医药卫生—临床医学]