尘螨抗原对支气管上皮细胞单核细胞趋化蛋白-1表达的影响  

Influence of house dust mite allergen Dermatophagoides pteronyssinus antigens on expression of monocyte chemoattractant protein-1(MCP-1) in bronchial epithelial cells

作　　者：孙宝清[1] 邬扬源[2] 汤葳[2] 钟南山[1] 

机构地区：[1]广州呼吸疾病研究所,510120 [2]香港大学医学院

出　　处：《中华微生物学和免疫学杂志》2005年第12期970-973,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的探讨屋尘螨(Dermatophagoides pteronyssinus,Derp)抗原对支气管上皮细胞单核细胞趋化蛋白-1(MCP-1)表达的影响。方法使支气管上皮细胞BEAS-2B暴露于系列不同浓度(0.02、0.2、2、20μg/ml)的Derp抗原24h至96h,分别观察各时间点细胞的表现,然后用酶联免疫法(ELISA)检测其细胞上清MCP-1的浓度表达。结果正常为未加Derp抗原,表现为单层细胞完全平铺;实验各组在抗原的刺激下,表现为随着浓度和时间的增加,细胞逐渐变瘦长,细胞间距逐渐增大;在无抗原刺激因素培养条件下的对照组,MCP-1释放水平很低,而在加入Derp抗原组,引起细胞分泌MCP-1蛋白水平的显著增加,并随着时间和浓度增加,MCP-1蛋白的表达水平呈上升趋势,特别是在高浓度组,即20μg/ml抗原组,各时间点MCP-1的表达差异均有统计学意义(P<0.01)。结论Derp抗原刺激气道上皮细胞,引起支气管上皮损伤和脱落等气道炎症反应,激发单核巨噬细胞产生大量的MCP-1,促成和加重气道炎症反应,因此认为MCP-1可能参与哮喘疾病的某些过程。Objective To investigate the effects of increasing exposure to Dermatophagoides pteronyssinus（Derp） antigens on the expressions of monocyte chemoattractant protein-1 （MCP-1） in bronchial epithelial cells （BEAS-2B）. Methods BEAS-2B was exposed to different concentrations of Derp antigens （0.02, 0.2, 2, 20 μg/ml） for 24 hours to 96 hours. The expression in different intervals was tested by ELISA. Results Complete flattening of single cell layer was observed in control group. In the experimental groups with antigen exposure, cell elongation as well as intercellular space enlargement were found and were positively related to exposure time or antigen concentration; the same profile was found on MCP-1 expression. Conclusion Derp antigen stimulates the airway epithelial cell, and causes airway inflammatory responses such as bronchial epithelial damage. It stimulates mononuclear macrophage to produce great amount of MCP-1, which may contribute to and deteriorate the airway inflammatory responses. It is believed that MCP-1 may have contribution to the pathogenesis of asthma.

关 键 词：屋尘螨 支气管上皮细胞 单核细胞趋化蛋白-1 哮喘 

分 类 号：R392[医药卫生—免疫学]