锂对沙土鼠全脑缺血后脑皮质区Bcl-2表达的影响  

The Effect of Preconditioning with lithium on The expressions of Bcl-2 Proteins in Gerbil after Global Ischemia

作　　者：施韬[1] 钱燕宁[2] 王斌[3] 

机构地区：[1]南京医科大学附属南京第一医院麻醉科,南京210006 [2]南京医科大学第一附属医院麻醉科,南京210029 [3]南京医科大学药理学系,南京210029

出　　处：《中国临床医学》2005年第6期972-974,共3页Chinese Journal of Clinical Medicine

基　　金：国家人事部出国留学人员资助项目(IA0001);南京医科大学创新基金项目(MO005)

摘　　要：目的:观察氯化锂预处理对沙土鼠脑缺血再灌注损伤后脑皮质区Bcl-2蛋白表达的影响。方法夹闭沙土鼠双侧颈总动脉5 min制备全脑缺血性脑损伤模型。12只沙土鼠随机分为实验组和对照组。实验组手术前连续5d给予氯化锂5 mmol／kg腹腔注射; 对照组以生理盐水代替氯化锂。分别于术后第1d将动物处死,取海马齿状回互包段制成石蜡切片,进行Bcl-2蛋白的免疫组化分析。结果:实验组脑皮质Bcl-2蛋白表达阳性面积(1452．37±418．04)μm2较对照组(1289．29±610．23)μm2明显增加(P<0．05)。结论氯化锂使脑皮质区Bcl-2蛋白表达上调,是其神经元保护作用的可能机制之一。Objective： To investigate the effect of preconditioning with lithium on the expressions of Bcl-2 proteins in gerbil after global ischemia. Methods： The transient ischemia model of gerbil global was established by clamping the bilateral common carotid arteries for 5 min. 12 gerbils were randomly divided into group LiCI （n= 6） and group Saline （n= 6）. Gerbils in group LiCI were injected intraperitoneally with lithium, 5mmol/kg, once a day for 5 consecutive days. Normal saline was used instead of lithium in group saline as vehicle control. All the gerbils underwent ischemia after preconditioning , then were killed 1 day after ischemia. The brains were made into paraffin sections and were used for immunohistochemistry against Bcl-2. Results： Compared with the vehicle control group [（ 1289. 29 ± 610. 23）μm^2 ], the Bcl-2 expression was upregulated in the cortex in the group LiCI [ （1452. 37 ± 418.04）μm^2 ], （P〈0. 05）. Conclusion： It might be one of the mechanisms underlying the neuroprotective effect of lithium that upregulates the expression of the Bcl-2 proteins.

关 键 词：脑缺血 预处理 锂 延迟性神经元死亡 Bccl-2 

分 类 号：R992[医药卫生—毒理学] R542.2[医药卫生—药学]