异搏定对α－石英细胞毒性拮抗作用的研究  

Resistance of verapamil to cytotoxicity of α-Quartz

作　　者：朱卫华[1] 胡继岳[1] 温和瑞[1] 王金晞[1] 

机构地区：[1]江西赣南师范学院,南京大学配位化学国家重点实验室

出　　处：《镇江医学院学报》1996年第1期9-10,共2页Journal of Zhenjiang Medical College

摘　　要：本文采用家兔肺泡巨噬细胞（ＡＭ）体外培养法，以细胞存活率、细胞内游离钙离子浓度（［Ｃａ２＋］１）为指标，研究了异搏定（Ｖｅｒａｐａｍｉ１）对二氧化硅细胞毒性的拮抗作用。结果表明：在含钙介质中，α－石英对巨噬细胞的毒性作用引起细胞存活率下降和胞浆游离Ｃａ２＋浓度的升高，并随α－石英剂量的增大和时间的延长作用加剧，这种效应可部分地被异搏定所抑制。但在无钙介质中，未观察到细胞存活率下降和胞浆游离Ｃａ２＋浓度升高的现象。This paper studied about the resistance effect of verapamil to the cytotoxicity of α- quartz by means of extcrnal culture of alveolar macrophage of rabbit with the indices of the cell's survival percent,eytoplasmic free Ca2+ concentration([Ca2+]2).The results are mainly as follows:In the Ca2+ -mcdium,the poisonous function ofαquartz to alvcolar macrophagc leads to the inereasc of[Ca2+]1.The higher the concetration of α-quartz dust suspension,or the longer the timce of action,the lower the cell's survival pereent and the grcater the increase of[Ca2+] 1.However,this effect can be partly inhibited by verapamil. But in the medium abscnce of Ca2+,the decreasc of cell's survival percent and the increasc of[Ca2+] 1 are not observed.

关 键 词：硅肺 异搏定 α-石英 细胞毒性 病理 

分 类 号：R598.2[医药卫生—内科学] R979.3[医药卫生—临床医学]