薯果类维生素C的分光光度法测定  

Spectrophotometric Determination for Vitamin C in the Tubers and Fruit

作　　者：王三艳[1] 王榆清[1] 赵福明[1] 黄锁义[1] 

机构地区：[1]右江民族医学院,广西百色533000

出　　处：《广东微量元素科学》2005年第12期41-43,共3页Trace Elements Science

基　　金：全国高等学校教学研究中心研究计划立项课题(BIA 010092-E 09)

摘　　要：为探讨分光光度测定薯果中维生素C的方法,在待测样品中加草酸处理过滤后于490nm作分光光度测定。结果表明,经分光光度扫描,生成物脎的吸收峰值为488～492nm,温度是测定过程中的重要因素。此法可用于薯果的维生素C测定,较化学方法简便。To discuss the method of measuring vitamin C in the tubers and fruit by spectrophotometer, was added oxalic acid to the await measuring sample to deal with into the even thick liquid, then used spectrophotometry for measuring it at 490 nm after filtering. The results show that through the spectrophotometer scaning, the peak value of the osazone is admitted as 488 ～ 492 nm. The temperature is the important influence factor in the course. It concludes this method can be used in the survey of vitamin C in the tubers and fruit, more simple and more convenient than the chemical method.

关 键 词：分光光度法 山药 维生素C 

分 类 号：Q946.6[生物学—植物学]