红毛五加多糖对辐射损伤小鼠的保护作用及其机制  

Protective effect of Acanthopanax giraldii Harms polysaccharides on radiation injury in mice and its mechanism

作　　者：鞠洋[1] 骆勤[1] 党月兰[2] 

机构地区：[1]兰州大学第二医院药剂科,730030 [2]兰州大学药学院药理学教研室

出　　处：《中华放射医学与防护杂志》2005年第6期533-536,共4页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的研究红毛五加多糖(AGP)对辐射损伤小鼠的保护作用及其机制。方法小鼠腹腔注射(ip)AGP125～250mg/kg,60Coγ射线全身均匀照射,观察小鼠30d存活率,检测AGP对外周血WBC、PLT、网织红细胞数、骨髓有核细胞数、血清ALT、AST、SOD、全血GSH-PX活性、血清和肝MDA含量以及肝、脾DNA、RNA含量的影响。结果辐射损伤后小鼠体重减轻、脱毛明显、尾部出现多个出血斑,30d存活率明显降低;外周血WBC、PLT、网织红细胞数、骨髓有核细胞数明显减少,ALT、AST活性增强,SOD、GSH-PX活性降低,MDA含量增加,DNA、RNA含量减少。AGP可促进上述指标的恢复。结论AGP对辐射损伤小鼠有明显保护作用,其机理可能与抗氧化作用及减轻DNA、RNA损伤有关。Objective To study the protective effect of Acanthopanax giraldii Harms polysaeeharides （ AGP）on radiation injury in mice and its mechanism. Methods The mice were treated with ip injection of AGP （125-250 mg/kg） and exposure to whole-body irradiation with ^60Co γ-rays. The white blood celI（WBC）,blood platelets（PLT）, reticulocyte counts, the number of nucleated bone marrow celI（NNBMC）, the content of MDA in the liver and serum, the content of DNA and RNA in the liver and spleen, and activities of ALT, AST, SOD, GSH-PX in the serum and blood were measured. Results After irradiation, the general status of the mice was changed as showed by hair deprivation, body weight reduction, blood spots appearance on the tail, and so on. The numbers of WBC, PLT, reticulocytes and NNBMC all reduced remarkablely. MDA contents and activities of ALT,AST increased obviously, and DNA, RNA contents and activities of SOD, GSH-PX decreased evidently afterir radiation. AGP could accelerate restoration of all these indexs. Conclusion AGP has protective effects on irradiated mice, the mechanism of which might be related with antioxidation.

关 键 词：红毛五加多糖 辐射损伤 外周血象 酶活性 核酸 抗氧化作用 

分 类 号：R285.5[医药卫生—中药学]