淫羊藿总黄酮对成骨细胞中OPG和RANKL mRNA基因表达影响的实验研究  

Effect of Herba Epimedii Flavone on Expression of OPG and RANKL in Rat Osteoblasts

作　　者：刘亦恒[1] 臧洪敏[1] 张海英[2] 陈君长[1] 

机构地区：[1]西安交通大学第二医院骨科,西安710004 [2]西安交通大学医学院解剖教研室,西安710061

出　　处：《中药材》2005年第12期1076-1078,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:探讨淫羊藿总黄酮对大鼠成骨细胞中骨保护素(OPG)和核因子κB受体激活因子配体(RANKL)mRNA基因表达的影响。方法:体外培养成骨细胞,分别加入含0·1、1、10、100ng/ml淫羊藿总黄酮(HEF)的培养液,48h及96h后分别提取细胞总mRNA,用RT-PCR方法分析OPG/RANKL的mRNA的表达,进行半定量分析。结果:培养48h后,与对照组比较,HEF增强了OPGmRNA的表达,有显著性差异(P<0·05或P<0·01),VOPG/VRANKL比值增大;96h后,HEF明显增强了OPGmRNA的表达,各浓度组与对照组相比,均有显著性差异(P<0·05或P<0·01),各浓度组VOPG/VRANKL比值增大,有显著性差异(P<0·05或P<0·01)。结论:淫羊藿总黄酮可能通过增加成骨细胞OPG的表达来抑制破骨细胞的分化和成熟,从而抑制骨吸收,达到治疗骨质疏松症的目的。Objective： To explore the effect of Herba Epimedii Flavone （HEF） on expression of OPG and RANKL in rat osteoblasts. Methods： Osteoblasts were obtained from new born rat calvaria by digestive enzymes. The different HEF concentrations（0. 1,1, 10,100 ng/ml）were added into the culture medium. Total mRNA was prepared from osteoblasts after 48 h and 96 h, and mRNA express/on of OPG and RANKL were detected by RT-PCR. Results： The mRNA expression of OPG in osteoblasts significantly increased compared with that of the control group, and the VOPG/VRANKL ratio was deceased markedly in the treatment of 10 ng/ml group after 48 h. After 96 h, the mRNA expression of OPG significantly increased and the VOPG/VRANKL ratio was also decreased in the treatment group compared with that of the control group. Conclusion： HEF suppress the differentiation and maturate of osteoblast. Osteoporosis can be prevented and cured by HEF.

关 键 词：淫羊藿总黄酮 成骨细胞 OPG RANKL 细胞培养 

分 类 号：R68[医药卫生—骨科学] R285.5[医药卫生—外科学]