变异银环蛇的蛇毒及DNA分析研究  

Analysis of Snake Venom and DNA Variety of a Aberrance Bungarus Multicinctus Blyth

作　　者：顾少菊[1] 唐上明[1] 孔天翰[1] 

机构地区：[1]广州医学院广州蛇毒研究所,广州510182

出　　处：《现代临床医学生物工程学杂志》2005年第6期484-486,共3页Journal of Modern Clinical Medical Bioengineering

摘　　要：目的比较变异与正常银环蛇蛇毒及DNA的差异,为进一步研究银环蛇在自然界的生存、进化、分类,提供分子生物学的研究基础.方法①利用聚丙烯酰胺凝胶电泳(SDS-PAGE)分析蛇毒蛋白的组分;②利用随机引物扩增(Random Amplified Polymorphic DNA,RAPD)的方法对1例变异银环蛇和10例华南常见银环蛇的DNA进行扩增.结果SDS-PAGE结果发现变异银环蛇毒的蛋白组分与正常银环蛇基本相同,但在43KD大小的一条蛋白带含量明显减少;RAPD扩增的DNA条带有明显差异.结论①变异银环蛇的蛇毒蛋白组分含量发生改变;②该变异银环蛇与正常银环蛇DNA差异显著.Objective To analysis and identify the differences between the aberrance and normal Bungarus Multicinctus Blyth in venom and DNA.This paper provides that materials for research snake classification and evolution in nature. Methods ①Using SDS-PAGE electrophoresis analyed the aberrance Bungarus multicinctus Blyth venom; ②Using random amplified the aberrance Bungurus multicinctus Blyth＇s DNA and 10 normal Bungarus multicinctus Blyth＇s DNA amplified,and using software SPSS12.0 statisical comparison the amplified bands. Results ①SDS-PAGE electrophoresis proved that the aberrance Bungarus multicinctus Blyth venom hade some differences from the normal. The amplified bands had distinct differences. Conclusions Analysis the result, the Bungurus multieinctus Blyth＇s DNA arises aberrance.

关 键 词：变异银环蛇 随机引物扩增 

分 类 号：R996.3[医药卫生—毒理学]