花背蟾蜍蝌蚪变态期角膜发育过程中Ⅰ型胶原的免疫组化彩色图像分析  

Color Image Analysis on Immunohistochemical Staining of Type I Collagen in the Corneal Development of Bufo raddei Larvae During Metamorphosis

作　　者：刘怀金[1] 张丽[2] 王子仁[2] 

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070 [2]兰州大学生命科学学院,兰州730000

出　　处：《实验动物科学与管理》2005年第4期6-9,共4页Laboratory Animal Science & Administration

基　　金：国家自然科学基金资助项目(30170109)

摘　　要：应用彩色图像分析技术对Ⅰ型胶原的免疫组化彩色图像分析可以弥补单纯应用定性指标或形态学指标的不足。花背蟾蜍蝌蚪在变态期角膜发育过程中随着Ⅰ型胶原的增多,内、外角膜逐渐愈合。Ⅰ型胶原的表达存在增龄变化,可以推测Ⅰ型胶原是构成角膜基质的主要成分,对建立和维持角膜支架有重要作用。Using the technology of immunohistochemical staining and color image analysis to investigate the spatiotemporal pattern and age-related changes of type Ⅰ collagen expression in the corneal development of Bufo raddei larvae during metamorphosis. Results showed that the inner and outer cornea combined together into mature cornea during metamorphosis. Both corneal epithelium and corneal stroma become thickened with progressively increase of the age. The analysis of immunohistochemical staining by color image analysis found that type Ⅰ collagen expressed widely during the period, existing in Bowman＇s membrane, Descemet＇ s membrane, corneal stroma and endothelium. Espeacially, a progressively change in corneal stroma was observed. Conclusion The defect of qualitative index may be remedied by color index in the quantitative immunohistochemical image analysis of type Ⅰ collagen. The inner and outer cornea combine together gradually with type Ⅰ collagen increasing in the corneal development of Bufo raddei larvae during metamorphosis. Type Ⅰ collagen, which is the main component in the increased collagens, expresses progressively with age. Type Ⅰ collagen may be the important component in corneal stroma, and it plays an important role in the construction and maintaining of the corneal stroma.

关 键 词：花背蟾蜍蝌蚪 角膜发育 Ⅰ型胶原 免疫组化 图像分析 

分 类 号：R392.11[医药卫生—免疫学] Q954.53[医药卫生—基础医学]