鼠奇异变形杆菌的鉴别诊断  

Identification of Proteus mirabilis in Laboratory Mice

作　　者：高正琴[1] 王春玲[1] 邢进[1] 贺争鸣[1] 高翔[2] 邢瑞昌[1] 

机构地区：[1]中国药品生物制品检定所国家实验动物质量检测中心,北京100050 [2]南京大学模式动物研究所国家遗传工程小鼠资源库,南京210032

出　　处：《实验动物科学与管理》2005年第4期46-49,共4页Laboratory Animal Science & Administration

摘　　要：目的对一株小鼠奇异变形杆菌进行了鉴定,为实验动物细菌检测、鉴别诊断提供参考依据。方法通过培养特性、菌落形态、染色和系列生化试验等检查,对疑似沙门氏菌的小鼠分离菌株进行初步鉴定,并采用血清凝集试验对小鼠分离菌株作进一步鉴定。结果通过表型生物学特性鉴定,并结合血清学诊断鉴别方法,确证该小鼠分离菌株为奇异变形杆菌。结论尽管检出的奇异变形杆菌不是SPF动物所必须排除的病原菌,但仍需指出:该菌为条件致病菌,对实验动物和研究人员均有潜在的危害,对科学实验也可能造成不利影响,故应引起高度重视。Objective To identify a strain of P. mirabilis isolated from laboratory mice which may provide an useful reference for bacterial detecting and identifying in laboratory animals. Methods The suspicious strain of Salmonella spp isolated from SPF mice was identified by cultural characteristics, colony morphology, staining, motility and biochemical testing, and was further identified with the slide agglutination test. Results The results of cultural characteristics, colony morphology, staining, biochemical testing, and slide agglutination test showed that the isolates was a strain of P. mirabilis but not Salmonella spp. Conclusion Although SPF mice are allowed to be P. mirabilis carriers in our country, it is worthy to state that P. mirabilis is an conditional pathogen, which may cause some potential harm to researchers and laboratory animals, and also can cause certain adverse effect to scientific experiments. It is still to be considered as an important affair because of the special use of the carriers.

关 键 词：小鼠 奇异变形杆菌 鉴定 

分 类 号：Q939.11[生物学—微生物学]