RP-HPLC测定复方蛇床子洗剂中蛇床子素的含量  

Determination of Osthol in Compound Fructus Cnidium Lotion by RPHPLC

作　　者：魏桂林[1] 彭肇强[1] 刘大伟 

机构地区：[1]赣南医学院附属医院 [2]赣州市药品检验所,江西赣州341000

出　　处：《赣南医学院学报》2005年第6期755-756,共2页JOURNAL OF GANNAN MEDICAL UNIVERSITY

摘　　要：目的:建立RP-HPLC外标法测定复方蛇床子洗剂中蛇床子素的方法.方法:采用Diammsil C18色谱柱(4.6mm×250mm,5μm),乙睛-水(65∶35)为流动相,检测波长322nm.以氯仿萃取制剂中的蛇床子素.结果:蛇床子素在0.04716～0.4716μg的范围内,峰面积(Y)与浓度(X)的线性关系良好,回归方程为Y=7968.28X+11.652(r=0.9985,n=6).加样回收率是96.45%(RSD=0.85 %,n=5),且精密度、稳定性、重现性都很好.结论:本方法的专属性、准确性、重现性好,可用于该制剂的质量控制.Objective： An RP-HPLC method was established for the quantitative determination of osthol in compound fructus cnidium lotion. Methods：A Diammsil C18 column（4.6mm × 250mm, 5μm） was used with acetonitrile - water（65 ： 35） as the mobile phase, the detective wavdength was 322nm. To use chloroform to draw osthd from compound fructus cnidium lotion. Results： The calibration curves was linear in the range of 0. 04716-0.4716μg for osthol （r= 0. 9996）. The average recovery and RSD was separately 96.45% and 0.85%. The precision, stability, and reproducibility of the experiment were very good. Conclusion：The method was specific, accurate and precise. It could be used for the quality control of compound fructus cnidium lotion.

关 键 词：RP-HPLC 测定 复方蛇床子洗剂 蛇床子素 

分 类 号：R284.1[医药卫生—中药学]