鸡肉中喹乙醇、卡巴多及其代谢物的残留检测  

Determination of olaquindox,carbadox and carbadox metabolites residues in chicken muscle

作　　者：殷居易[1] 倪梅林[1] 寿成杰[2] 房科腾[1] 谢东华[1] 李佐卿[1] 

机构地区：[1]宁波出入境检验检疫局技术中心食品安全分中心,浙江宁波315012 [2]宁波大学食品科学学院,浙江宁波315000

出　　处：《中国兽药杂志》2006年第1期11-15,共5页Chinese Journal of Veterinary Drug

摘　　要：建立了测定鸡肉组织样品中喹乙醇、卡巴多以及喹噁啉-2-羧酸残留量的高效液相色谱法。鸡肉样品中的药物用乙腈与乙酸乙酯的混合液(1∶1,V/V)提取,经过浓缩、净化,用甲醇定容进行检测。色谱拄为Atlantis C18柱,流动相由甲醇、水和乙酸钠缓冲液(pH 4.6)组成,采用梯度洗脱程序;检测波长为320和380 nm。喹乙醇、卡巴多及噁喹啉-2-羧酸在0.05~1.0μg/mL浓度范围内,药物峰面积与浓度值呈良好的线性关系,其相关系数分别为0.997 5、0.997 9、0.998 2。空白鸡肉中添加药物浓度为0.05、0.1、0.2和0.5μg/?时,喹乙醇的回收率为(70.6±3.1)%^(87.5±3.6)%;卡巴多为(73.2±3.7)%^(91.5±2.7)%;喹噁啉-2-羧酸为(71.9±4.3)%^(86.6±3.5)%。喹乙醇、卡巴多和喹噁啉-2-羧酸的最低检测限分别为0.05、0.015、0.025μg/mL。In this article an HPLC method is described for the determination of olaquindox, carbadox and quinoxaline-2-carboxylic acid （QCA）residues. The drugs in the chicken muscle were extracted by a mixture of acetonitrile -ethyl acetate（ 1：1 ） and diluted in methanol for HPLC determination. The chromatographic column was Atlantis C18. Mobile phase was a mixture of methanol - water and acetate buffer. Samples were detected with a UVdiode-array detector at 320 nm and 380 nm. The response for olaquindox, carbadox and QCA was linear in the range of 0.1 - 1.0 μg/mL with the correlation coefficient of 0. 9975, 0. 9979, 0. 9982, respectively. When the blank muscle samples were fortified with 3 drugs at 0. 05,0.1 and 0.2 μg/g, the mean recoveries were （70.6 ± 3.1）%,（80.8±3.6）%,（87.5±3.6）% forolaquindox, （73.2±3.7）% ,（83.0±4.3）%,（91.5±2.7）% for carbadox, （71.9 ±4.3 ） %, （78.2 ±4.6） %, （86.6 ±3.5） % for QCA. The minimum detection limit of olaquindox, carbadox and QCA residues in chicken muscle was 0.05, 0. 015, 0. 025 μg/mL, respectively.

关 键 词：喹乙醇 卡巴多 喹噁啉-2-羧酸(QCA) 高效液相色谱(HPLC)法 DAD检测器 

分 类 号：S859.84[农业科学—临床兽医学]