鸡组织中氯霉素残留的气相色谱-微电子捕获检测方法研究  

Determination of chloramphenicol residue in chicken tissues by GC-μECD

作　　者：周金慧[1] 张素霞[2] 沈建忠[2] 孙志文[3] 陈玲[2] 

机构地区：[1]农业部蜂产品质量监督检验测试中心,北京100093 [2]中国农业大学动物医学院,北京100094 [3]北京市兽药监察所,北京100012

出　　处：《中国兽药杂志》2006年第1期16-19,共4页Chinese Journal of Veterinary Drug

摘　　要：建立了鸡肌肉和鸡肝脏组织中氯霉素残留的气相色谱-微电子捕获检测方法。用乙酸乙酯提取样品中的药物，正己烷脱脂，乙酸乙酯液-液分配，氮气吹干后，用Sylon BFT衍生化，进配有微电子捕获检测器（μECD）的气相色谱仪测定，外标法定量。鸡肌肉组织在0．1、0．5、1．0μg／kg三个添加水平，平均回收率为90．2％～94．3％，日内变异系数在4．5％-11．6％之间，日间变异系数在7．8％～14．3％之间，检测限0．05μg/kg，定量限0．10μg/kg。鸡肝脏组织在0．2、0．5、1．0μg/kg三个添加水平，平均回收率为82．9％～90．8％，日内变异系数在7．0％～11．2％之间。日间变异系数在7．9％～14．5％之间，检测限0．10μg/kg，定量限0．20μg/kg。A method of gas chromatography with microcell electron capture detector （GC-μLECD） was developed to determine chloramphenicol residues in chicken muscle and liver tissues. The drug in tissues were extracted with ethyl acetate. The extract was defatted with hexane and purified with ethyl acetate by liquid-liquid distribution,evaporated to dryness with nitrogen gas. After derivatization by adding sylon BFY, tolune and water were added immediately to the sample. The organic layer was carefully removed. 3μL processed liquid samples were determined by GC-μECD. Recoveries ranged from 82.9% to 94.3% with coefficients of variation from 4.5% to 14.5%. The limit of detection is 0.05 μg/kg in muscle, and 0.1 μg/kg in liver.

关 键 词：氯霉素 气相色谱-微电子捕获检测 残留 鸡组织 

分 类 号：S859.84[农业科学—临床兽医学]