硫酸软骨素-阳离子表面活性剂缔合物微粒体系的共振散射光谱研究及分析应用  

Resonance Scattering Spectral Study of Cationic Surfactant-Chondroi-tin Sulfate Association Particle Systems and Its Analytical Application

作　　者：蒋治良[1] 邹明静[1] 梁爱惠[2] 沈星灿[1] 

机构地区：[1]广西师范大学资源与环境学系,桂林541004 [2]桂林工学院材料与化学工程系,桂林541004

出　　处：《化学学报》2006年第2期111-116,共6页Acta Chimica Sinica

基　　金：广西自然科学基金(No.0575042);广西高校百名中青年学科带头人资助计划基金;桂林工学院科研启动基金资助项目.

摘　　要：在适宜的pH条件下,硫酸软骨素(CDS)可与溴代十四烷基吡啶(TPB)等阳离子表面活性剂形成稳定的缔合物微粒.激光散射法测得(TPBn-CDS)m缔合物微粒的平均粒径为737.6nm.TPB-CDS,十四烷基二甲基苄基氯化铵[(TDMBA)-CDS],氯代十六烷基吡啶[(CPCM)-CDS],十六烷基三甲基溴化铵[(CTAB)-CDS]缔合物微粒体系的共振散射光谱研究表明,TPB-CDS和TDMBA-CDS缔合物微粒体系均在360nm处存在较强的共振散射峰;CPCM-CDS和CTAB-CDS缔合物微粒体系均在340nm处存在较强的共振散射峰.TPB,TDMBA,CPCM和CTAB共振散射测定硫酸软骨素的线性范围分别为0.02～24.0,0.02～8.0,0.02～8.0,0.02～8.0μg?mL-1;检出限分别为0.013,0.011,0.013,0.016μg?mL-1.研究了共存物质对TPB共振散射测定CDS的影响,方法的选择性较好,用于合成样品和注射液样品中CDS测定,结果令人满意.Under the conditions of suitable pH, chondroitin sulfate （CDS） was reacted with cationic surfactant, such as tetradecyl pyridinium bromide （TPB）, tetradecyl dimethyl benzyl ammonium chloride （TDMBA）, cetyl pyridinium chloride monohydrate （CPCM） and cetyl trimethyl ammonium bromide （CTAB）, to form stable association microparticles. The laser scattering indicates that the average diameter of （TPBn-CDS）m association particles was about 737.6 nm. The resonance scattering spectra of the four association particle systems including TPB-CDS, TDMBA-CDS, CPCM-CDS and CTAB-CDS were studied. The strong resonance scattering peaks of TPB-CDS and TDMBA-CDS were all at 360 nm, while those of CPCM-CDS and CTAB-CDS systems emerged at 340 nm. To the systems of TPB, TDMBA, CPCM and CTAB, CDS concentration in the range of 0.02～24.0, 0.02～8.0, 0.02～8.0 and 0.02～8.0 lμg·mL^-1 was proportional to the intensity with the detection limits of 0.013, 0.011, 0.013 and 0.016 μg·mL^-1, respectively. The effect of coexistent substance on the determination of CDS was studied, owing to the high sensitivity of TPB-CDS, it has been applied to the determination of CDS in synthetic samples and injection ones with satisfactory results.

关 键 词：硫酸软骨素 阳离子表面活性剂 缔合物微粒 共振散射 

分 类 号：O647.2[理学—物理化学]