复方甘草片中甘草酸、异甘草苷、异甘草素的测定  

Quantitative Determinations of Glycyrrhizic Acid,Isoliquiritin and Isoliquiritigenin in Compound Liquorice Tablets

作　　者：刘晓娜[1] 高彦华[1] 乔喜芹[2] 李强[2] 范玉玲[2] 

机构地区：[1]东北林业大学植物药工程研究中心,哈尔滨150040 [2]哈尔滨商业大学制药工程系

出　　处：《黑龙江医药》2006年第1期1-3,共3页Heilongjiang Medicine journal

摘　　要：目的：建立复方甘草片中甘草酸、异甘草苷和异苷草素的含量定量分析的高效液相色谱(HPLC)方法。方法：采用色谱柱ODS-C_(18)(4．6mm×250mm,5μm),甘草酸流动相为甲醇：冰醋酸：水(70：1：30)；检测波长254nm。异甘草苷与异甘草素在同一条件下检测,其流动相为甲醇：冰醋酸：水(50：2：50)；检测波长346nm；三者流速均为1ml /min；进样量为10μl；柱温为25℃。结果：复方甘草片中甘草酸的含量为7．51mg/g,异甘草苷和异甘草素的含量分别为3．01mg/g,0．448mg/g,上述3种组分的平均回收率分别为98．32%,99．57%和98．59%。结论：本方法可作为复方甘草片中的甘草酸、异甘草苷和异甘草素的含量质量控制的一种有效方法。Objective： HPLC was used to detemaine the contents of glycyrrhizic acid, isoliquiritin and isoliquiritigenm in compound liquorice tablets quantitatively.Methods： The HPLC analysis of glycyrrhizic acid was performed with the duent comprising of methanol： acetic acid： water（ 70： 1： 30）and detection wavelength was 254nm.The HPLC analysis of isoliquiritin and isoliquiritigenin was performed simultaneously with the eluent comprising of methanol： acid： water（ 50： 2： 50）and detection wavelength was 346nm.The flow rate of HPLC analysis was 1.0ml/min and the injection was 10μl.Results： The result showed that the content of glycyrrhizic acid was 7.51mg/g, and the content of isoliquiritin and isoliquiritigenin were 3.01mg/g and 0.488mg/g respectively.Their average recoveries were 98.32%, 99.57% and 98.59%, respectively.Conclusion：The method can be used for quality control of compound liquorice tablets.

关 键 词：高效液相色谱 复方甘草片 甘草酸 异甘草苷 异甘草素 

分 类 号：R284[医药卫生—中药学]