板蓝根对脂多糖刺激鼠释放炎性细胞因子的影响  

Effect of Radix Isatidis on LPS-stimulated Release of Inflammatory Cell Factors of Mice

作　　者：刘云海[1] 尹雄章[1] 谢委[1] 石初晶 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉市430030

出　　处：《中国药房》2006年第1期18-20,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(39170877;39870872);卫生部科学研究基金资助项目(98-2-110)

摘　　要：目的探讨板蓝根抗内毒素活性部位F022对脂多糖(LPS)刺激鼠单核细胞释放炎性细胞因子的影响。方法取BALB/C小鼠腹腔内单核细胞,实验设计为6组。其中,实验组根据F022浓度分为1%、0.5%、0.25%、0.125%4组,分别加入板蓝根F022部位液后再加入LPS液;LPS阳性组仅加入LPS液;阴性组加入1%F022液。之后检测细胞培养上清液中3种炎性细胞因子肿瘤坏死因子-α(TNF-α)、白细胞介素6(IL-6)和一氧化氮(NO)水平。结果LPS可刺激鼠单核细胞过度释放炎性细胞因子TNF-α、IL-6和NO;与阳性组比较,实验组炎性细胞因子水平降低,且呈剂量依赖性。结论板蓝根抗内毒素活性部位F022对LPS刺激鼠单核细胞过度释放炎性细胞因子具有抑制作用。OBJECTIVE： To investigate the effect of the active site F022 of Radix Isatidix antiendotoxin on the LPS-stimulated release of inflammatory cell factors in monocytes of mice. METHODS： Monocytes samples taken from the abdominal cavities of BALE/ C mice were divided into 6 groups, the trial group were divided into 1%, 0.5% , 0.25% and 0.125% 4 groups, which added with Radix Isatidis F022 sample solution and LPS solution; The positive group were treated with LPS solution; The negative group were added with 1% F022 solution. Thereafter the levels of TNF-α, IL-6 and NO of the 3 kinds of inflammatory cell factors cultured in the clear supernatant liquid were determined. RESULTS： LPS could stimulate monocytes of mice to excessively release inflammatory cell factors including TNF-α, IL - 6 and NO. Compared with the positive group, the levels of inflammatory cell factors, in the trial group reduced and which was dose dependent. CONCLUSION： The active site F022 of Radix Isatidix antiendotoxin has inhibitory action on LPS- stimulated excessive release of inflammatory cell factors of in monocytes of mice.

关 键 词：板蓝根 脂多糖 肿瘤坏死因子-Α 白细胞介素6 一氧化氮 

分 类 号：R285.5[医药卫生—中药学]