人类胚胎冷冻、复苏及移植的临床应用研究  

Clinical application of transfer cryopreservation and thawing in the human embryos

作　　者：周从容[1] 李荣荣[1] 陈淼鑫[1] 罗锦[1] 陈卓[1] 赵淑云[1] 

机构地区：[1]贵阳医学院附属医院生殖医学中心,550004

出　　处：《贵州医药》2006年第1期11-13,共3页Guizhou Medical Journal

摘　　要：目的探讨冷冻胚胎的临床应用,提高试管婴儿的成功率。方法对人类4～8细胞期胚胎进行慢速冷冻法保存、快速复苏法解冻及移植,比较冷冻前后优质胚胎率及移植后的妊娠情况。结果 (1)35例患者共126枚胚胎复苏。冷冻前后平均优质胚率分别为69．2％(128／185)与23．2％ (43／185),两者相比差异有显著意义(P<0．01)。(2)胚胎冷冻、复苏移植后临床妊娠率为20％(7／ 35);患者累积临床妊娠率为45．6％(47／103)。(3)自然周期移植临床妊娠率26．7％;激素替代周期移植临床妊娠率15％,两者差异无统计学意义。结论采用冻融胚胎技术可以提高一次取卵的总妊娠率。冷冻、复苏可降低优质胚胎率,但有较满意的临床妊娠率。Objective To analysis frozen- thawed human embryos and clinical pregnancy rates after embryo transplant. Methods A slow- freezing and rapidthawing protocol were used in 4-8 cells human embryos then comparing good quality embryo rates and clinical pregnancy rates after embryo transplant. Results （1） 126 embryos of 35 patients were frozen and thawed. The average good quality embryo rates of prefreezing and thawing were 69.2% （128/185） and 23. 2% （43/185） respectively. There was significant difference between two groups （P〈0. 01）. （2） The clinical pregnancy rate of human embryo m the freezing-thawing patients was 45. 6%（47/103）. （3） The clinical pregnancy rate in natural cycle cases showed not difference with that in controlled endometrium cycle cases （P〉 0. 05）. Conclusion there is a lower good quality embryo rate of frozen- thawed embryos but it have a good clinical pregnancy, rate.

关 键 词：胚胎冷冻 胚胎复苏 胚胎移植 妊娠率 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]