薄膜干卵石蜡切片免疫酶染法抗原稳定性及试剂条件的研究  

Studies on the Antigen Stability of Immunoenzymatic Staining Technique Using Dry Egg Section on PVC-membrane

作　　者：陈河[1] 何加玕 顾启章[1] 

机构地区：[1]杭州医学高等专科学校

出　　处：《浙江师大学报（自然科学版）》1996年第2期87-89,共3页Journal of Zhejiang Normal University(Natoral Sciences)

摘　　要：应用薄膜干卵石蜡切片免疫酶染法同步检测不同条件保存后抗原的稳定性，结果表明：抗原不脱蜡分别置室温与冰箱后，存放１年（阳性率均为９３．３３％）与制备３天（阳性率为９６．６７％）相比，无显著性差异（Ｘ２＝０．３５，Ｐ＞０．０５）；同时，在室温与冰箱中存放１年的两组抗原在染色程度上也未见显著性差异（Ｕ＝１．４８，Ｐ＞０．０５）；提示：不脱蜡的薄膜干卵抗原，其活性不受时间及室温变化的影响，具有高度的稳定性。用相同方法检测不同试剂条件对实验的影响，表明：实验中如能采用适当的试剂浓度（Ａ蛋白为１∶１６，ＤＡＢ为１∶４），可以提高实验的稳渡性和结果的准确性。Immunoenzymatic staining technique(IEST) using dry egg section on PVC-membrane was employed for detering antigen stability that were reserVed in different conditions. The results showed:to put the antigen sections respectively at room temperature and in the refrigerator with wax for a year, both the peitive rates were 93. 33% and the staining degree of the two groups was similar,in comparison with the antigen section that were mode three days(the positive rate being 96. 67%),there was no sisnificant statistic difference(P>0. 05). The results indianaed that the actigen activity using dry egg section on PVC-membrane with wax was not affected by changes of the time and the temperature, and it possessed higher stability.

关 键 词：免疫酶染色 血清学 抗原 稳定性 血吸虫病 

分 类 号：R532.210.4[医药卫生—内科学]