节瓜抗枯萎病变异体离体筛选研究初报  

Study on selection in vitro of the mutants with Fusarium wilt resistance in chieh-qua

作　　者：何晓明[1] 谢大森[1] 彭庆务[1] 穆利霞[1] 

机构地区：[1]广东省农科院蔬菜研究所,广东广州510640

出　　处：《广东农业科学》2006年第1期49-51,共3页Guangdong Agricultural Sciences

基　　金：广东省自然科学基金项目(010110);广东省农业攻关重大专项(2003A2010301);广州市农业科技招标项目(GK0401002)

摘　　要：利用镰刀菌酸（FA）为胁迫剂，以节瓜不定芽为筛选材料，进行节瓜抗枯萎病变异体离体筛选技术的研究，初步试验结果表明FA胁迫的适宜浓度为75～100mg／L。经过筛选的细胞系对FA的抗性较未经筛选的细胞系明显增强。初步建立了节瓜抗性变异体离体筛选体系，获得了抗FA变异体再生植株的自交种子。Selection in vitro for the mutants with Fusarium acid （FA） resistance in chieh- qua was carried out. The result showed that the suitable concentration of FA for selection was 75～100 mg/L. The resistance to FA of the selected - colonies was higher than that of tile un-selected. The systcm of sclcetion in vitro for the resistant mutants in Chieh-qua was established. The inbreeding seeds of mutants were obtained.

关 键 词：节瓜 枯萎病 离体筛选 

分 类 号：S642.903.6[农业科学—蔬菜学]