多发性骨髓瘤中p16、p15基因甲基化及其mRNA表达的研究  

Study on hypermethylation and mRNA expression of p16 and p15 gene in multiple myeloma

作　　者：梁新悦[1] 姜玉珍[1] 徐文[1] 刘建惠[1] 

机构地区：[1]吉林大学第二医院血液科,吉林长春130041

出　　处：《临床荟萃》2006年第1期10-13,共4页Clinical Focus

基　　金：吉林省科技厅基金资助项目(20010535)

摘　　要：目的探讨p16、p15基因的高甲基化及其mRNA转录阻抑与多发性骨髓瘤的发病和进展之间的关系。方法采用甲基化敏感的限制性内切酶联合聚合酶链反应方法(REP法),检测了28例多发性骨髓瘤患者骨髓细胞p16、p15基因5'端启动子区的甲基化状态,采用逆转录聚合酶链反应(RT-PCR)方法检测多发性骨髓瘤患者p16、p15基因mRNA表达情况。结果p16、p15基因启动子区过度甲基化率分别为57.14%(16/28)、64.28%(18/28),53.57%(15/28)患者同时存在p16、p15基因启动子区过度甲基化。大多数存在甲基化的患者不表达p16、p15基因mRNA。结论p16、p15基因的高甲基化与多发性骨髓瘤的发病有关,p16、p15基因甲基化与其转录阻抑高度相关。Objective To illustrate the role of hypermethylation of p16 and p15 genes and their mRNA expression in the pathogenesis of multiple myeloma （MM）. Methods By using restriction enzymeralated polymeras chain reaetion（REP） and reverse transcript polymeras chain reaetion（RT-PCR）, the methylation status in both p16 and p15 genes and their mRNA expression were analysed. Results Methylation was found in 57.14% （16/28） for p16, 64. 28%（18/28） for p15 and 53.57% （15/28） for both genes in MM. The mRNA of p15 and p16 genes were not expressed in most patients who have p15 and p16 genes hypermethylation. Conclusion The results suggested that hypermethylation of p16 and p15 genes plays an important role in MM tumourigenesis. There is a strong correlation between methylation and transcriptional repression of p16 and p15 genes.

关 键 词：多发性骨髓瘸 基因 p16 基因 p15 甲基化 

分 类 号：R551.3[医药卫生—血液循环系统疾病]