口服感染昆虫纯化重组杆状病毒  

PURIFICATION OF RECOMBINANT BACULOVIRUS WITH POLYHEDRON BY PER OS INFECTION OF INSECT LARVAE

作　　者：施先宗[1] 陈曲侯[1] 王珣章[1] 庞义[1] 龙綮新[1] 

机构地区：[1]华中师范大学昆虫学研究所,中山大学昆虫学研究所生物防治国家重点实验室

出　　处：《华中师范大学学报（自然科学版）》1996年第2期207-211,共5页Journal of Central China Normal University：Natural Sciences

摘　　要：构建可使重组杆状病毒产生多角体的重组转移载体质粒，并将共转染产物喂昆虫幼虫，收集典型病变幼虫血淋巴进行空斑分析，发现子代病毒中约９０％为重组病毒．再进行一轮空斑纯化即可获得纯净的重组病毒，较之传统的空斑纯化方法，节省了人力、物力和时间．Constuction of transfer plasmids that help recombinant baculoviruses produce polyhedra facilitates the purification of recombinant baculoviruses. Insect larvae were per os infected in this experiment with the resultant of the cotransfection of viral DNA and Plasmid DNA, and the hemolymph of diseased larvae were collected. Plaque assay showed that about 90% prgeny viruses produced in the larvae were the expected recombinant baculovirus. Another turn of plaque purification gave 100% recombinant baculoviruses. It decreases the cost and the time by this way compared to the conventional plaque assay procedure.

关 键 词：口服感染 昆虫幼虫 杆状病毒 纯化效率 重组病毒 

分 类 号：Q78[生物学—分子生物学]