植物多糖对日本血吸虫DNA疫苗pVIVO2-IL12-Sj23增效作用的研究  

Studies on Effect Enhancement of the Schistosoma japonicum DNA Vaccine pVIVO2,IL12,Sj23 by Vegetal Polysaccharides

作　　者：冯清[1] 胡俊峰[2] 陈涵[2] 楼建林[2] 甘燕[3] 胡媛[3] 石佑恩[3] 

机构地区：[1]华中科技大学化学系 [2]华中科技大学同济医学院临床医学 [3]华中科技大学同济医学院寄生虫学教研室,武汉430030

出　　处：《中国寄生虫学与寄生虫病杂志》2005年第6期401-403,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：湖北省重点科技攻关项目(批准号:2001AA301C31和2003AA301D13)~~

摘　　要：目的以提取天然香菇菌多糖和茶叶多糖混合物为佐剂,探讨混合型植物多糖对血吸虫疫苗的保护性免疫增效作用。方法构建日本血吸虫重组质粒pVIVO2-IL12-Sj23。BALB/c小鼠随机分为3组,每组10只。A组每鼠股四头肌注射生理盐水100μl为对照,B组每鼠注射pVIVO2-IL12-Sj23 100μg,C组每鼠注射pVIVO2-IL12-Sj23 100μg加等量多糖混合物。小鼠接种后第4周以日本血吸虫尾蚴40±2条经皮肤攻击感染,感染后6周剖杀,计算减虫率及每克肝组织减卵率,同时用ELISA测定血清中特异性抗体水平。结果C组减虫率和每克肝组织减卵率分别为64.3%和79.9%,B组则分别为45.5%和58.3%,B、C两组的差异有统计学意义(P<0.05)。B、C两组IgG水平均较A组显著升高(P<0.05),但B、C两组IgG抗体水平的差异无统计学意义(P>0.05)。结论以天然香菇菌多糖和茶叶多糖混合物为佐剂,对日本血吸虫重组质粒pVIVO2-IL12-Sj23的免疫效果有一定的增效作用。Objective To enhance the immunogenieity of the recombinant pVIVO2-IL12-Sj23 vaccine of Schistosoma japonicum by using mixed vegetal polysaccharides as adjuvant. Methods The plasmid pVIVO2-IL12-Sj23 was constnedtcd, 3 groups of BALB/C mice were injected intramuscularly with normal saline （Group A）, pVIVO2-IL12-Sj23 plasmid DNA （B）. and pVIVO2-IL12-Sj23 plus mixed vegetal polysaecharides. （C） respectively, and challenged with S.japonicum cercariaciae on the 4th week after imnumization. Mice were killed to calculate the worm reduetion rule and egg redtution rah, in liver tissue on the 6th week after infection. Before and 4 weeks after immunization blood samples were collected. Result,; The worm redtution rate and egg reduction rate were 64.3% and 79.9%, respectively in group C, 45.5% and 58.4%, respeclivcly in group B, showing a remarkable difference between them （P〈0.05）. ELISA analysis showed a signifieantly higher level of lgG speeific for Sj234 weeks after vacvination in groups B and C （P〈0.05）. Howcver, there was no signifieant difference in IgG level betwaeent groups C and B （P〉0.05）. Conclusion When mixed vegetal polysaccharides are used as adjuvant, the eftect of the vaccine pVIVO2-IL12-Sj23 can be considerably enhanced.

关 键 词：mll发虫病 pVIVO2-IL12-Sj23 疫苗 茶叶多糖 香菇菌多糖 住剂 

分 类 号：R383.24[医药卫生—医学寄生虫学]