慢性阻塞性肺疾病患者外周血T淋巴细胞亚群与肺功能的相关性  

Correlation between T lymphocyte subgroup in peripheral blood and pulmonary function in patients with chronic obstructive pulmonary disease

作　　者：任自力[1] 朱黎红[1] 吴凌康[1] 傅骞[1] 

机构地区：[1]杭州市中医院呼吸科,浙江省杭州市310007

出　　处：《中国临床康复》2005年第47期64-65,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的通过对慢性阻塞性肺疾病T淋巴细胞亚群与肺功能测定,了解两者间的相关性。方法①选择2001-11/2002-11杭州市中医院呼吸科呼吸科住院及门诊就诊慢性阻塞性肺疾病患者50例为患者组。其中缓解期患者20例,男13例,女7例;急性发作期30例,男23例,女7例。选择与慢性阻塞性肺疾病患者年龄匹配的同期本院健康体检者10人为对照组,男6例,女4例。纳入对象均对检测项目知情同意。②采用流式细胞仪检测淋巴细胞群中标记的CD4细胞百分比,CD8细胞百分比,并计算CD4与CD8的比值。③采用肺功能仪测定肺功能(第1秒用力呼气量、第1秒用力呼气量占预计值的百分比、最大用力呼气中段流量占预计值百分比)。④组间计量资料差异比较小样本,正态,方差齐采用t检验;小样本,非正态或方差不齐采用t’检验),相关性处理采用直线相关分析。结果慢性阻塞性肺疾病患者50例和健康者10人均进入结果分析。①在急性发作期及缓解期慢性阻塞性肺疾病患者外周血CD4细胞所占百分比明显低于对照组(t=4.87,9.70,P<0.05,0.01),而急性发作期慢性阻塞性肺疾病患者外周血CD8细胞所占百分比明显高于对照组和缓解期患者(t=2.72,2.07,P<0.05),发作期慢性阻塞性肺疾病患者外周血CD4/CD8和最大用力呼气中段流量明显低于缓解期(t=3.04,t’=16.9,P<0.05,0.01),第1秒用力呼气量占预计值百分比与缓解期差异不明显。最大用力呼气中段流量与急性期CD8细胞百分比呈显著负相关(r=-0.431,P<0.05),与CD4/CD8呈显著正相关(r=0.564,P<0.01)。结论CD8细胞与慢性阻塞性肺疾病患者急性发作期小气道功能密切相关。AIM： To determine the T lymphocyte subgroup and puhnonary function in patients with chronic obstructi＇,e puhnonary disease, and investigate their correlation. METHODS： （1）Fifty inpafients and outpatients with chronic obstructive puhnonary disease （patient group） were selected from the Department of Respiratory Medicine, Hangzhou Hospital of Traditional Chinese Medicine between November 2001 and November 2002, including 20 cases at remission （13 males and 7 females） and 30 cases at exacerbation （23 males and 7 females）. Another 10 healthy physical examinees （6 males and 4 females） matched by age with the patients with chronic obstructive puhnonary disease in the hospital were taken as the control group. （2）The CD4 and CD8 percentages in labeled lymphocyte group were detected with flow cytometer, and then the CD4/CD8 ratio was calculated. （3）At the same time, the pulmonary function （forced expiratory volume in the first second）, percentage of foreed expiratory volume in the first second to the predicted value, the percentage of maximal forced expiratory flow during middle half of forced vital capacity to the predicted value） were determined with puhnonary function meter. The intergroup difference of enumeration data were analyzed with t test or t＇ test, and the correlation was analyzed with linear correlation analysis. RESULTS： All the 50 patients with chronic obstruetive pulmonary disease and 10 healthy subjects were involved in the analysis of results. The pereentage of CD4 in peripheral blood was obviously lower in patients with chronic obstructive puhnonary disease at exacerbation and remission than in the control group （t=4.87, 9.70, P 〈 0.05, 0.01）, but the percentage of CD8 in peripheral blood was obviously higher in the patients at exacerbation than in the control group and the patients at remission （t =2.72, 2.07, P 〈 0.05）. The CD4/CD8 in peripheral blood and maximal forced expiratory flow during middle half of forced vital capacity in the patients a

关 键 词：肺疾病 慢性阻塞性 T淋巴细胞亚群 呼吸功能试验 

分 类 号：R563[医药卫生—呼吸系统]