玉竹提取物A对小鼠单核细胞体外诱生血栓素B2分泌量的影响  

Influence of extraction A of polygonatum odoratum on the secretion of in vitro induced thromboxane B2 in the monocytes of mice

作　　者：赵良中[1] 赵良化 蒋辛[3] 潘兴瑜[1] 

机构地区：[1]锦州医学院免疫与病原生物学教研室,辽宁省锦州市121001 [2]庄河市第四人民医院内一科,辽宁省大连市116400 [3]锦州医学院药理学教研室,辽宁省锦州市121001

出　　处：《中国临床康复》2005年第47期91-93,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的观察小鼠单核细胞在体外经内毒素刺激分泌血栓素B2的情况,及不同浓度玉竹提取物A对其分泌量的影响。方法实验于2004-06/2004-08在锦州医学院免疫实验室进行。选择普通级健康雄性昆明小鼠36只,随机分为正常对照组、模型对照组、玉竹提取物A500m g/L组、玉竹提取物A1000m g/L组、玉竹提取物A1500m g/L组,玉竹提取物A2000m g/L组共6组,每组6只。36只小鼠摘眼球取血,分离外周血单核细胞,分置24孔培养板中,每孔0.5m L。每组设6个平行孔,大肠杆菌内毒素为终浓度。正常对照组每孔加完全16400.5m L;模型对照组同样每孔加完全16400.5m L;同时4个实验组的单核细胞内分别加入相应浓度的玉竹提取物A;另取两个24孔培养板,每孔只加单核细胞悬液1m L。上述分离之单核细胞培养3d后换液1次,除正常对照组外,将10m g/L的大肠杆菌内毒素10μL加入其余5组,共育2h;同时取只加单核细胞悬液的后两板,每组加大肠杆菌内毒素浓度分别为0.01,0.1,1,10,100,400mg/L,各10μL,共育2h。然后收集每孔上清液,放免分析法测定上清液中血栓素B2含量。观察体外给予不同浓度大肠杆菌内毒素,对血栓素B2分泌量的影响;不同浓度玉竹提取物A对同一浓度大肠杆菌内毒素刺激的血栓素B2分泌量的影响。结果36只小鼠均进入结果分析。①大肠杆菌内毒素维持在0.01,0.1,1,10mg/L时,其刺激小鼠外周血单核细胞合成血栓素B2水平呈剂量依赖关系,即随大肠杆菌内毒素浓度增加,血栓素B2合成水平亦升高(800,950,1180,1500ng/L),大肠杆菌内毒素为10m g/L时,刺激血栓素B2合成速率达峰值;当内毒素刺激量增至100,400m g/L时,血栓素B2合成水平不再升高,而呈下降趋势(1100,900ng/L)。②当用10m g/L的大肠杆菌内毒素分别刺激除正常对照组外的其余5组的单核细胞时,模型对照组血栓素B2分泌量明显高于正常对照组[(1382±98),(823±78)ng/L,t=10.93,P<0.01],4�AIM： To observe the secretion of thromboxane B2 （TXB2） in monocytes of mice induced by endotoxin in vitro, and the influence of extraction A of polygonatum odoratum （EA-PAOA） on the TXB2. METHODS： The experiment was carried out in the immunological laboratory of Jinzhou Medical College between June and August 2004. Thirty-six common healthy Kunming mice were randomized into 6 groups with 6 mice in each group： normal control group, model control group, EAPAOA 500, 1 000, 1 500 and 2 000 mg/L groups. The mice were killed and their blood samples were collected by removing eyeballs. Monocytes in peripheral blood were separated to be cultivated in 24 wells, 0.5 mL for each well. There were 6 parallel wells in each group, bacillus coli endotoxin was the terminal concentration. Each well in the normal control group and model control group was added by 0.5 mL complete 1 640 culture medium, and EA-PAOA of corresponding concentration was added to the monocytes in the four experimental groups. Another 24-well culture plate was used, each well was added by 1 mL monocyte suspension. The liquids were changed once after the separated monocytes mentioned above were cultured for 3 days, and then 10 μL bacillus coli endotoxin （10 mg/L） was added into the other 5 groups except the normal control group, and cocultured for 2 days. Meanwhile, the latter two plates, in which only monocyte suspension was added, were selected, the concentrations of bacillus coli endotoxin in the 6 groups were 0.01, 0.1, 1, 10, 100, 400 mg/L, 10 p,L for each group for 2 hours. And then the supernatant of each well was collected to detect the TXB2 content with radioimmunoassay. The influences of in vitro given bacillus coli endotoxin of different concentrations on the secretion of TXB2 were observed. The effects of EA- PAOA of different concentrations on the secretion of TXB2 stimulated by bacillus coli endotoxin of same concentration were also observed. RESULTS： All the 36 mice were involved in the analysis of results. （1） When th

关 键 词：中草药/药理学 玉竹 内毒素类/代谢 血栓烷B2/代谢 

分 类 号：R285[医药卫生—中药学]